胞嘧啶脱氨酶/5-氟胞嘧啶基因治疗联合放疗对裸鼠子宫颈癌移植瘤生长抑制作用的研究  

Anticancer efficacy of combining cytosine deaminase/5-fluorocytosine gene therapy and radiotherapy on cervical carcinoma

作　　者：高晓林[1] 李清丽[1] 彭芝兰[1] 王和[1] 

机构地区：[1]四川大学华西第二医院妇产科

出　　处：《中华妇产科杂志》2005年第11期761-764,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨胞嘧啶脱氨酶(CD)/5-氟胞嘧啶(5-FC)基因治疗联合放疗对裸鼠宫颈癌移植瘤的生长抑制作用,以及CD/5-FC基因治疗与放疗之间是否具有协同作用。方法将24只鼠龄6～8周成功接种了HeLa细胞的BALB/C雌性裸鼠,随机分为对照组、基因治疗组、放疗组以及联合治疗组,每组6只,观察各组治疗前后的肿瘤体积、肿瘤生长抑制率及肿瘤体积增加倍数。结果(1)治疗21d后,基因治疗组裸鼠的肿瘤体积是(728±201)mm3,联合治疗组是(357±113)mm3,放疗组是(739±419)mm3,对照组是(1168±380)mm3,基因治疗组、联合治疗组、放疗组肿瘤体积均小于对照组,3组分别与对照组比较,差异有统计学意义(P<0.05);联合治疗组肿瘤体积小于基因治疗组及放疗组,前者分别与后两组比较,差异有统计学意义(P<0.05)。(2)放疗组的肿瘤生长抑制率为36.74%,基因治疗组为37.66%,联合治疗组为69.45%,联合治疗组明显高于放疗组及基因治疗组,前者分别与后两组比较,差异有统计学意义(P<0.05)。(3)联合治疗组平均肿瘤体积增加倍数为7.71倍,基因治疗组为19.16倍,放疗组为18.86倍,对照组为40.57倍。析因设计的方差分析结果,CD/5-FC基因治疗与放疗之间有交互作用(P=0.03),当与放疗联合使用时,CD/5-FC基因治疗的疗效大于其单独使用时所产生的疗效;当与CD/5-FC基因治疗联合应用时,放疗的疗效大于其单独使用时所产生的疗效。结论CD/5-FC基因治疗与放疗联合应用,对裸鼠宫颈癌移植瘤的生长具有抑制作用,并且两者之间具有协同作用。Objective To observe the effect of combining cytosine deaminase （CD）/5- fluorocytosine （5-FC） gene therapy with radiotherapy on cervical carcinoma in nude mice, and to explore if there is a synergistic effect between the two therapies. Methods HeLa cells were injected into the 24 female nude mice who were 6-8 weeks old. When the tumors grew to 4.0-7.0 mm in diameter, the mice were divided into 4 groups randomly ： control group, CD/5-FC gene therapy group, CD/5-FC gene therapy plus radiotherapy group, radiotherapy group. The volumes and inhibiting rates of the tumors were calculated. Results （1） The tumor volumes in CD/5-FC gene therapy group （728 ± 201 ） mm^3, CD/5-FC gene therapy plus radiotherapy group （357 ± 113） mm^3, radiotherapy group （739 ±419） mm^3 , were smaller compared with that in the control group （ 1168 ± 380） mm^3 , the difference was significant （ P 〈 0. 05 ） ; tumor volumes in CD/5-FC gene therapy plus radiotherapy group was significantly smaller compared with that in the CD/5-FC gene therapy group and radiotherapy group （ P 〈 0.05）. （2） The tumor-inhibiting rate of the radiotherapy group was 36. 74%, of the CD/5-FC gene therapy group was 37.66%, and of the CD/5-FC gene therapy plus radiotherapy group was 69.45%, the latter being significantly higher than that of the former two groups （ P 〈 O. 05 ）. （ 3 ） There was a synergistic effect between CD/5-FC gene therapy and radiotherapy. Conclusions There is a synergistic effect between CD/5-FC gene therapy and radiotherapy.CD/5-FC gene therapy combined with radiotherapy may be a good supplementary method for cancer synthetic treatment.

关 键 词：基因疗法 放射疗法 宫颈肿瘤 胞嘧啶脱氨酶 氟尿嘧啶 

分 类 号：R737.33[医药卫生—肿瘤]