钙离子阻断剂对大鼠肠上皮细胞损伤后整合素分布的影响  

The effect of calcium channel blocker on integrin distribution of intestinal epithelium cells undergoing injury in rats

作　　者：陈前[1] 秦环龙[1] 

机构地区：[1]上海交通大学附属第六人民医院外科,200233

出　　处：《中华普通外科杂志》2005年第11期721-723,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金(批准号30271286);上海市科委青年科技启明星跟踪计划(批准号02QMB1406)资助项目

摘　　要：目的观察钙离子阻断剂对肠上皮细胞(IEC)缺血缺氧损伤后细胞内钙含量、整合素分布及凋亡的影响。方法实验分为对照组(A1组),模拟缺氧组(B1组),模拟缺血组(C1组),模拟缺氧缺血组(D1组)及加入维拉帕米后的相应对照组;用流式细胞仪(FCM)检测IEC凋亡率,用激光聚焦显微镜技术(LSCM)观察胞内钙含量、整合素α3,α5,β1,β2,β5分布改变。结果模拟缺血缺氧损伤后,B1、C1、D1组胞内钙含量升高,IEC凋亡率上升,整合素α3,α5,β1,β5向顶层漂移,尤以D1组更显著;加用钙离子阻断剂后,降低了胞内钙含量,阻止整合素α3,α5,β1,β5顶层漂移趋势,其变化与细胞凋亡减少相一致。结论细胞内钙超载可导致细胞内整合素分布改变,凋亡率增加,钙离子阻断剂能降低细胞内钙含量,抑制整合素亚型的顶层漂移,减少IEC凋亡率。Objective To study the influence of calcium channel blocker（CCB） on intracellular integrin distribution of IEC suffering from ischemia and anoxia injury. Methods Model rats were divided into A1 group （control）, B1 group （anoxia） , C1 group （ischemia） , D1 group （ischemia and anoxia） ; A2 group （ A1 treated by 2 mg/L verapamil ） ; B2 group （ B1 treated by 2 mg/L verapamil ）, C2 group （ C1 treated by 2 mg/L verapamil ）, D2 group （ D1 treated by 2 mg,/L verapamil ）. Apoptosis of IECs were measured; intracellular calcium amount and distribution of integrins α3,α5,β1,β2,β5 were determined by LSCM. Results Intracellular calcium and apoptosis rate （AR） increased after ischemia and anoxia injury, integrins α3,α5,β1,β2,β5 moved to the apical of membrane, especially in group D1. The effects were reversed by CCA. Conclusions Intracellular calcium overload induces distribution alterations of integrins α3,α5,β1,β2,β5, increases apoptosis rate. These effects could be reversed by CCB.

关 键 词：肠黏膜 凋亡 钙离子阻断剂 整合素 

分 类 号：R574[医药卫生—消化系统]