兔VX2肿瘤细胞系的建立及其生物学特性的观察  

Establishment and characterization of a rabbit tumor cell line VX2

作　　者：刘险峰[1] 任乐荣[1] 苏广彦[1] 刘玉琴[1] 顾蓓[1] 董继红[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院,北京100005

出　　处：《中华病理学杂志》2005年第10期661-663,共3页Chinese Journal of Pathology

基　　金：国家重点基础研究计划资助项目(2002CB5131)

摘　　要：目的建立兔VX2肿瘤细胞系,观察其生物学特性。方法采用小块法对兔VX2肿瘤进行原代培养,体外传代观察,传代40次以上对培养细胞进行形态学观察、组织化学染色、细胞周期检查、核型分析、兔及裸鼠移植。结果新建立的兔VX2肿瘤细胞,呈多角形、短梭形,电镜下细胞内可见张力纤维、细胞间可见桥粒,细胞角蛋白阳性,体外连续培养10个月,传代70次以上,细胞倍增时间为34.5h,细胞周期测定G1期为69.3%,G2期为5.6%,S期为25.1%。染色体为亚三倍体核型,众数为58～62条。同种移植成瘤率100%,裸鼠移植成瘤率100%,无支原体污染。结论兔VX2肿瘤细胞系来源于兔鳞状细胞癌,可用于兔(较大动物)的肿瘤实验研究。Objective To establish a rabbit tumor cell line and to characterize its biological parameters. Methods VX2 tumor tissue was used for the primary culture in vitro. After 40 passages, the cell morphology, CK expression （immunohistochemical staining）, cell cycle, karyotype and tumorigenecity in rabbits and nude mice were investigated. Results The newly established cell line VX2 was maintained in continuous culture for over 70 passages in 10 months. Morphologically, VX2 cells were polygonal to short spindled. Tonal fibril and tight junction were found under the electron microscope. CK was positive. The cell cycle analysis showed 69. 3% in G1 phase, 5.6% in G2 phase and 25. 1% in S phase. The population doubling time was 34. 5 hours. The chromosomal analysis showed a hypotriploidy with a median chromosome number of 58 -62. The tumorigenecity in rabbits and nude mice were both 100%. Conclusion The established VX2 cell line derived from rabbit squamous carcinoma could serve as a model system for experimental oncology in the rabbit.

关 键 词：兔 肿瘤细胞 培养的 肿瘤标记 生物学 

分 类 号：R73-3[医药卫生—肿瘤]