Tiam1对结直肠癌细胞生物学特性的影响  

Tiam1 expression correlates with the biological behavior of human colorectal carcinoma cells

作　　者：刘莉[1] 许岸高[2] 杨玉芳[1] 丁彦青[1] 

机构地区：[1]南方医科大学南方医院病理科,广州510515 [2]广东省惠州市中心人民医院

出　　处：《中华病理学杂志》2005年第10期664-668,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目(30370649);广东省重大专项基金资助项目(2003A308401);广东省自然科学基金资助项目(04006711)

摘　　要：目的探讨Tiam1(Tlymphomainvasionandmetastasis)对结直肠癌细胞株生物学特性的影响。方法用逆转录聚合酶链反应方法检测Tiam1基因在结直肠癌细胞株中的表达,筛选Tiam1不表达的细胞株;将Tiam1/C1199HAcDNA导入内源性不表达Tiam1基因的人结直肠癌HT29细胞株中,G418筛选抗性克隆,免疫组织化学和Western蛋白印迹法鉴定转染后Tiam1基因在细胞中的表达,利用四甲基偶氮唑盐(MTT)法检测Tiam1对细胞增殖的影响,体外侵袭实验检测Tiam1对结直肠癌侵袭转移的影响。结果Tiam1基因在高转移的LoVo和SW620中高表达,在低转移的HT29中不表达,在SW480和HCT116细胞表达相应较低。通过7d的连续比较,HT29/mock和HT29/Tiam1两组的细胞增殖能力差异有统计学意义(F=10.512,P=0.003)。Tiam1基因的导入使结直肠癌细胞的增殖能力明显增强,体外侵袭转移能力显著增加,HT29/Tiam1穿过细胞为(88.6±9.2)个/200倍视野,HT29/mock为(46.8±3.4)个/200倍视野,二者的差异具有统计学意义(t=9.54,P<0.01)。结论Tiam1基因具有促进结直肠癌细胞增殖的能力,Tiam1与结直肠癌的侵袭转移密切相关,Tiam1表达可作为结直肠癌侵袭转移过程中一个有价值的指标。Objective To investigate whether the Tiam1 gene expression enhances the invasive and metastatic capabilities of colorectal carcinoma cells. Methods Endogenous expression of Tiam1 in five colorectal carcinoma cell lines was investigated by RT-PCR. Tiam1/C1199HA cDNA was transfected into HT29, a colorectal carcinoma cell line without endogenous Tiam1 expression. RNA and protein expression of Tiam1 gene in the transfectants were detected by RT-PCR, immunohistochemistry and Western blot respectively. The biological behaviors of the transfectants were investigated by MTI＂ and in-vitro invasion assays. Results Tiam1 gene was highly expressed in LoVo and SW620 cells. Low level expression was seen in HCT116 and SW480 and no expression was found in HT29. Transfection of Tiam1 significantly increased the proliferation of HT29 cells along with markedly enhanced in-vitro invasion and metastasis. Conclusions Tiam1 gene plays an important role in the invasion and metastasis of colorectal carcinoma. It may be a useful marker for metastasis of colorectal carcinoma.

关 键 词：结直肠肿瘤 肿瘤转移 转染 

分 类 号：R735.3[医药卫生—肿瘤]