Receptor-binding cancer antigen expressed on SiSo cells can be detected in metastatic lymph nodes from gastrointestinal cancers  

作　　者：Kawin Leelawat Surang Engprasert Supathip Tujinda Cheepsumon Suthippintawong Munechika Enjoji Manabu Nakashima Takeshi Watanabe Vijittra Leardkamolkarn 

机构地区：[1]Department of Surgery [2]Department of Pharmacognosy [3]Department of Pathology [4]Department of Medical Bioregulation [5]Department of Anatomy

出　　处：《World Journal of Gastroenterology》2005年第38期6014-6017,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by the Thailand Research Fund in the Royal Golden Jubilee Program

摘　　要：AIM： To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells （RCAS1） in metastatic lymph nodes from gastrointestinal cancer. METHODS： Metastatic lymph nodes from gastrointestina cancer were detected for RCAS1 by immunohistochemica staining and mRNA in situ hybridization.RESULTS： A total of 102 metastatic lymph nodes from bile duct, gastric, colon, and pancreatic cancer were investigated for RCAS1 expression. The immunoreactivity of RCAS1 was identified in 100% of metastatic lymph nodes. Both local and distant metastatic lymph nodes showed RCAS1 expression. On the contrary, specimens of non-cancerous lymph nodes were negative for RCAS1. The result of mRNA in situ hybridization was also confirmed by the finding of immunohistochemical staining. RCAS1 mRNA was detected in all tumor cells that metastasized to lymph nodes. CONCLUSION： All metastatic lymph nodes express RCAS1 in tumor cells at both protein and mRNA levels, and RCAS1 should be used as a complementary factor for identification of metastatic lymph nodes from gastrointestinal cancers.AIM: To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) in metastatic lymph nodes from gastrointestinal cancer.METHODS: Metastatic lymph nodes from gastrointestinal cancer were detected for RCAS1 by immunohistochemical staining and mRNA in situ hybridization.RESULTS: A total of 102 metastatic lymph nodes from bile duct, gastric, colon, and pancreatic cancer were investigated for RCAS1 expression. The immunoreactivity of RCAS1 was identified in 100% of metastatic lymph nodes. Both local and distant metastatic lymph nodes showed RCAS1 expression. On the contrary, specimens of non-cancerous lymph nodes were negative for RCAS1.The result of mRNA in situ hybridization was also confirmed by the finding of immunohistochemical staining. RCAS1 mRNA was detected in all tumor cells that metastasized to lymph nodes.CONCLUSION: All metastatic lymph nodes express RCAS1 in tumor cells at both protein and mRNA levels,and RCAS1 should be used as a complementary factor for identification of metastatic lymph nodes from gastrointestinal cancers.

关 键 词：RCAS1 Gastrointestinal cancer Lymph node metastasis 

分 类 号：R735[医药卫生—肿瘤]