六种纳米载银无机抗菌剂的体外细胞毒性比较  

Comparison of the cytotoxicity in vitro among six types of nano-silver base inorganic antibacterial agents

作　　者：张富强[1] 佘文珺[1] 傅远飞[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔修复科,200011

出　　处：《中华口腔医学杂志》2005年第6期504-507,共4页Chinese Journal of Stomatology

基　　金：上海市教育发展基金资助项目(200201)

摘　　要：目的通过分析6种纳米载银无机抗菌剂不同浓度稀释液的体外细胞毒性,初步评价纳米载银无机抗菌剂的生物安全性。方法将载银磷酸锆(FUMAT T200-4)、载银磷酸复盐(HN300)、载银氢氧化钠锆(Novaron)、载银磷酸锆(康旺)、载银二氧化硅(MOD)、载银磷酸锆(SR1000)配置成100、50、25、12.5g/L4种不同质量浓度的稀释液,采用噻唑蓝(MTT)比色法检测各浓度稀释液对小鼠成纤维细胞L-929的体外细胞毒性并进行比较。结果6种纳米载银无机抗菌剂的高浓度稀释液对小鼠成纤维细胞L-929均有毒性,随着浓度下降,细胞毒性亦下降,当浓度≤25g/L时已无毒性。对6种纳米载银无机抗菌剂的体外细胞毒性进行比较,结果显示其安全性为FUMAT T200-4、康旺、SR1000>HN300>Novaron、MOD。结论纳米载银无机抗菌剂FUMATT200-4、康旺及SR1000的生物安全性较好,具有作为口腔材料应用的可行性。在应用时,其浓度应≤25g/L,以确保对人体的安全性。Objective To evaluate the biocompatibility of nano-silver base inorganic antibacterial agents and compare the cytotoxicity in vitro among six types of nano-silver base inorganic antibacterial agents. Methods FUMAT T200-4, HN300, Novaron, Kangwang, MOD and SR1000 were diluted to different concentrations, such as 100 g/L,50 g/L,25 g/L and 12. 5 g,/L. The cytotoxicity in vitro of these agents on rat＇ s fibroblast was assayed with MTT method. And the grades of cytotoxicity were compared. Results High concentrations of nano-silver base inorganic antibacterial agents had cytotoxic effects on rat＇ s fibroblasts L-929. As the concentration decreased, the cytotoxicity decreased. No cytotoxic effects were observed at or below the concentration of 25 g/L. FUMAT T200-4, Kongwang and SR1000, with the carrier of phosphate zirconium, had less cytotoxity than the others. Conclusions Nano-Silver base inorganic antibacterial agents, such as FUMAT T200-4, Kangwang, SR1000, have good biocompatibility. And they have the possibility of clinical application. The safe concentration of these agents is at or below 25 g/L.

关 键 词：牙科材料 生物相容性 细胞毒性 体外 

分 类 号：R783.1[医药卫生—口腔医学]