β1整合素反义寡核苷酸对人胰腺癌裸鼠皮下移植瘤的治疗作用  

Effect of integrin-β1 antisense oligodeoxynucleotides on human pancreatic cancinoma transplanted subcutaneously in nude mice

作　　者：黄涛[1] 高军[1] 冯延平[1] 常青[1] 胡均[1] 秦仁义[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科,湖北武汉430030

出　　处：《中国普通外科杂志》2005年第11期809-812,共4页China Journal of General Surgery

摘　　要：目的探讨β1整合素反义寡核苷酸(ASODN)对裸鼠人胰腺癌移植瘤生长和基因表达的影响。方法建立裸鼠人胰腺癌皮下移植瘤模型,随机分为3组:对照组(皮下注射脂质体和转染液)、非特异序列(RODN)组(皮下注射RODN、脂质体和转染液)和ASODN组(皮下注射ASODN、脂质体和转染液)。治疗后计算抑瘤率和肿瘤缩小率,检测肿瘤中β1整合素mRNA和蛋白表达。结果RODN组、ASODN组抑瘤率分别为4.7 5%和7 2.7 0%,ASODN组肿瘤缩小1 0.9 1%。与其他两组比较,ASODN组裸鼠肿瘤中β1整合素mRNA和蛋白表达水平明显降低。结论靶向β1整合素的反义寡核苷酸对人胰腺癌裸鼠皮下移植瘤的生长具有一定的抑制作用,可能为胰腺癌治疗提供新的方法。Objective To investigate the effect of integrin-β1 antisense oligodeoxynucleotide （ASODN） on human pancreatic cancinoma transplanted subcutaneously in nude mice, Methods The models of human pancreatic cancinoma transplanted subcutaneously were established in nude mice, then divided randomly into 3 groups and different treatment was given respectively （control group, random oligodeoxynucleotide group and ASODN group）, After treatment, the weight of nude mice and tumor volume were observed, and the tumor growth inhibitory rate and the tumor response rate were calculated. The expressions of integrin-β1 mRNA and protein in tumor tissue were determined by RT-PCR and Western-blot, Results The tumor growth inhibitory rate in the random oligodexynucleotide group and the ASODN group was 4. 75% and 72, 70% , respectively, The tumor decrease rate of the ASODN group was 10. 91%. The expression level of integrin-β1 mRNA and protein was decreased in the ASODN group compared with other 2 control groups, Conclusions Our findings suggest that integrin-β1 antisense oligodeoxynucleotides result in marked inhibition of human pancreatic cancinoma growth in nude mice. It may be a novel treatment approach for human pancreatic carcinoma,

关 键 词：胰腺肿瘤/治疗 寡核苷酸类 反义/治疗应用 肿瘤移植 

分 类 号：R735.9[医药卫生—肿瘤] Q555.7[医药卫生—临床医学]