Ang-2对血管内皮细胞内皮素分泌的影响及茶多酚的保护作用  

作　　者：韩磊[1] 李鸣皋[1] 马贵喜[1] 刘玉[1] 李靖[1] 蒙果[1] 刘昕[1] 

机构地区：[1]海军总医院海军航空潜水医学中心,北京100037

出　　处：《山东医药》2005年第32期19-20,共2页Shandong Medical Journal

基　　金：海军后勤科研基金资助项目(00-3321)

摘　　要：目的探讨血管紧张素-2(Ang-2)对血管内皮细胞(VEC)内皮素(ET)分泌的影响及茶多酚的保护作用。方法将培养的VEC分为4组,每组8个样本。空白对照组仅加入细胞培养液(Ⅰ组),Ⅱ组单纯加入Ang-2使终浓度为10-5mol/L,Ⅲ组在Ⅱ组的基础上加入茶多酚使终浓度为50mg/L,Ⅳ组使茶多酚终浓度为25mg/L,用放射免疫法测定Ang-2作用前、作用后0.56、、24h各组ET含量。结果Ⅱ组较Ⅰ组各时间ET含量显著增高(P<0.01),Ⅲ组在6h、24h较Ⅱ组,Ⅳ组在0.5h、6h、24h较Ⅱ组ET含量均有显著差异(P均<0.01)。结论Ang-2使VEC分泌ET的功能增强,茶多酚能显著抑制Ang-2促VEC分泌ET的作用,且低浓度优于高浓度茶多酚。Objective：To explore the effects of Angiotesin-2 （Ang-2） on vascular endothelial cells （VEC） secreting endothelin （ET） and protection of different concentration tee polyphenols. Methods： Cultured VEC were divided into 4 groups ：control group were only given cell culture medium （ Ⅰ group） ; Ⅱ group were given Ang-2 with the last concentration be 10-Stool/L; Ⅲ group were given tee polyphenols with the last concentration be 50mg/L at the base of Ⅱ group; Ⅳ group were given tee polyphenols with the last concentration be 25mg/L at the base of Ⅱ group. The ET content in supernatant of VEC before hypoxia and 0.5h after hypoxia ,6h after hypoxia as well as 24h after hypoxia were measured with radioimmunoassay. Results： The ET content of Ⅱ group is significantly higher than that of Ⅰ group （P,〈0.01） ; The ET content of I group and Ⅱ group at the time of 6h,24h were different （P〈0.01）;The ET content I group and Ⅳ group at the time of 0.5h,6h,24h were different （P 〈 0. 01）. Conclusion： Ang-2 can significantly increase the ET release of VEC . Tee polyphenols can decrease the VEC secreting ET induced by Ang-2 and the lower concentration tee polyphenols is better.

关 键 词：血管内皮细胞 内皮素 血管紧张素-2 茶多酚 

分 类 号：R331.36[医药卫生—人体生理学] R852.11[医药卫生—基础医学]