肝细胞生长因子对卵巢癌血管生成因子的影响及信号传导途径  

Hhepatocyte growth factor stimulate vascular endothelial growth factor secretion in ovarian cancer cell line and its signal transduction pathway

作　　者：王化丽[1] 张淑兰[1] 鲁艳明[1] 姜继勇[2] 朱晓钰[3] 曾伟利 

机构地区：[1]中国医科大学附属二院妇产科,110004 [2]大连市妇产医院 [3]大连市中心医院中心实验室 [4]大连市第四人民医院妇产科

出　　处：《中国妇产科临床杂志》2005年第6期435-438,共4页Chinese Journal of Clinical Obstetrics and Gynecology

摘　　要：目的研究肝细胞生长因子(HGF)对人卵巢癌SKOV3细胞血管内皮生长因子的影响及其信号传导机制。方法将不同浓度、不同作用时间的HGF和核转录因子(NFkB)抑制剂PDTC刺激培养的SKOV3细胞;应用逆转录-聚合酶链反应技术(RT-PCR)检测卵巢癌细胞血管内皮生长因子(VEGF)mRNA的变化;采用蛋白印迹方法检测卵巢癌细胞VEGF蛋白和核蛋白NFkB的变化。结果HGF促进卵巢癌细胞VEGF mRNA和蛋白的表达,且随时间和浓度增加作用增强,PDTC可以抑制HGF的刺激作用;HGF促进细胞核NFkB蛋白的活化,且随时间延长作用增强,于刺激1 h达高峰,PDTC可以抑制HGF对NFkB的活化作用。结论HGF通过NFkB途径在核酸和蛋白水平调节卵巢癌细胞分泌VEGF。Objective To observe the effects of hepatocyte growth factor （HGF） on vascular endothelial growth factor （VEGF） in ovarian epithelial cancer cells （SKOV3） and its signal transduction pathway. Methods The ovarian cancer cells was treated with HGF at different concentration and for varied time, or treated with Nuclear factor kB （NFkB） inhibitor PDTC. The expression level of VEGF mRNA in the cells was evaluated by relative quantitative reserve-transcription polymerase chain reaction （RT-PCR）. Western blot were used to examine VEGF protein and NFkB protein. Results HGF increased the expression of VEGF mRNA and protein in ovarian cancer cell line SKOV3 in dose- and time- dependant manner. Expression of nucleus NFkB protein was increased by HGF in a time- dependant manner and the peak appeared when the ceils were treated with HGF for 1 hour. However, PDTC significantly inhibited the expression of NFkB protein induced by HGF. Conclusion HGF modulates the expression of VEGF in ovarian cancer at mRNA and protein level via NFkB pathway.

关 键 词：卵巢肿瘤 肝细胞生长因子 肿瘤细胞培养的 血管内皮生长因子 

分 类 号：R737.31[医药卫生—肿瘤]