以虫草素和腺苷含量为指标优化蛹虫草人工栽培  

Optimization of Condyceps militaris Cultivation for High Production of Condycepin and Adenosine

作　　者：温鲁[1] 夏敏[2] 葛宜和[1] 刘金刚[1] 翁梁[1] 喻涟淮[1] 王帅[1] 沈芳[1] 蒋红[1] 

机构地区：[1]淮阴师范学院生物系,江苏淮安223300 [2]淮阴师范学院分析测试中心,江苏淮安223300

出　　处：《江苏农业学报》2005年第4期359-363,共5页Jiangsu Journal of Agricultural Sciences

基　　金：淮安市农业发展基金(HAN0221)

摘　　要：为提高人工栽培蛹虫草中主要活性成分的含量,以虫草素和腺苷含量为检测指标进行蛹虫草优化栽培研究,在采用Cm-1菌株、以20%豆粕为氮源、水料比为1.4的条件下,可获得子实体产量为每瓶42.2 g、子实体中虫草素含量为4.46 mg.g-1的栽培效果,虫草素含量超过了以蚕蛹为寄主的蛹虫草(2.83 mg.g-1),表明植物蛋白完全可以用作栽培蛹虫草的氮源,同时证实采收子实体后的培养基中仍含有大量虫草素,可作为提取虫草素的原料。To enhance the production of effective components of man-cultivated Cordyceps militaris, the optimized media with high yield of cordycepin and adenosine was investigated. In the media with 20% bean cake and 1.4 ： 1 ratio of H20 to dry weight, a strain of Cm^-1 could produce 4.46 mg · g^-1 of cordycepin in fruitbody, contrasting with 2.83 mg · g^-1 of cordycepin produced in the media with silkworm pupa. It is demonstrated that Cordyceps militaris can be cultivated in media with plant protein and the cultivated media may become resource of cordycepin.

关 键 词：蛹虫草 虫草素 腺苷 

分 类 号：S567.3[农业科学—中草药栽培]