Identification of a Positive Cis-Element Upstream of Human NKX3.1 Gene  

作　　者：An-Li JIANG 

机构地区：[1]Department of Biochemistry, Medical School of Shandong University, Jinan 250012, China

出　　处：《Acta Biochimica et Biophysica Sinica》2005年第11期773-778, ,共6页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.30470952);the Natural Science Foundation of Shandong Province(No.Y2004C26)

摘　　要：NKX3.1 is a prostate-specific homeobox gene related to prostate development and prostate cancer. In this work, we aimed to identify precisely the functional cis-element in the 197 bp region （from -1032 to -836 bp） of the NKX3.1 promoter （from -1032 to ＋8 bp）, which was previously identified to present positive regulatory activity on NKX3.1 expression, by deletion mutagenesis analysis and electrophoretic mobility shift assay （EMSA）. A 16 bp positive cis-element located between -920 and -905 bp upstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functional positive cis-element by EMSA. It will be important to further study the functions and regulatory mechanisms of this positive cis-element in NKX3.1 gene expression.NKX3.1 is a prostate-specific homeobox gene related to prostate development and prostate cancer. In this work, we aimed to identify precisely the functional cis-element in the 197 bp region （from -1032 to -836 bp） of the NKX3.1 promoter （from -1032 to ＋8 bp）, which was previously identified to present positive regulatory activity on NKX3.1 expression, by deletion mutagenesis analysis and electrophoretic mobility shift assay （EMSA）. A 16 bp positive cis-element located between -920 and -905 bp upstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functional positive cis-element by EMSA. It will be important to further study the functions and regulatory mechanisms of this positive cis-element in NKX3.1 gene expression.

关 键 词：human NKX3.1 cis-acting element positive regulation MUTAGENESIS 

分 类 号：R737.25[医药卫生—肿瘤]