大孔型纤维素硫酸钠-聚二甲基二烯丙基氯化铵微胶囊固定假丝酵母产脂肪酶  

Cultivation of encapsulated C. valida for producing lipase inmacro-porous NaCS-PDMDAAC microcapsules

作　　者：季艳艳[1] 姚善泾[1] 张俊[1] 关怡新[1] 林东强[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,浙江杭州310027

出　　处：《化工学报》2005年第11期2162-2165,共4页CIESC Journal

基　　金：国家自然科学基金项目(20276065).~~

摘　　要：In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semicontinuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.

关 键 词：脂肪酶 细胞固定化 大孔型微胶囊 纤维素硫酸钠-聚二甲基二烯丙基氯化铵微胶囊 假丝酵母 

分 类 号：Q939.7[生物学—微生物学]