空间诱变肿瘤细胞和生物工程细胞的染色体实验观察  

Observation of chromosomes of tumor cells and bio-engineering cells muted by outer space

作　　者：靳耀英[1] 向青[1] 徐波[1] 徐梅[1] 李红艳[1] 高福云[1] 刘伟[1] 石玉惠[1] 李静[1] 

机构地区：[1]中日友好医院临床医学研究所,北京100029

出　　处：《中国康复医学杂志》2005年第11期820-823,共4页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:摸索空间环境诱变后肿瘤细胞和生物工程细胞的染色体制备方法,分析染色体数目、结构的异常变化。方法:将肿瘤细胞和生物工程细胞无源搭载于第18颗返回式卫星和第20颗返回式卫星舱内,分别经18天空间飞行,回收后制作染色体标本,并对其结果进行染色体核型分析。结果:经太空诱变后的生物工程细胞染色体畸变率为:环状占10.5%,无着丝断片占36.75%,三射、四射体占13%。经太空诱变后的肿瘤细胞染色体畸变率为:非整倍体占68%,多倍体占32%。结论:①建立了一套本实验室太空诱变后肿瘤细胞和生物工程细胞的染色体制备方法。②发现空间诱变后的肿瘤细胞无明显染色体异常。生物工程细胞CHO(dhfr-)染色体畸变率增加。Objective： To investigate the method of chromosome preparation of tumor cells and bio-engineering cells mutated by outer space, and to analyze the abnormal changes of number and structure of chromosomes. Method： Tumor cell lines and bio-engineering cell lines were carried in the No. 18 and No. 20 recoverable satellite under the environment in which the cell living systems were maintained in passive carrying. After 18 days of spaceflight , the karyotype of chromosomes from surviving cells was analyzed. Result： The abnormal chromosome, ate of bio-engineering cells muted by outer space were： ring chromosome, 10.5%; acenericfragment, 36.75%; triceneric and fourcentric, 13%. The abnormal chromosome rate of tumor cells muted by outer space were： aneuploid, 68%, muhiploid, 32%. Conclusion： ①A series of laboratorial methods of chromosome preparation of tumor cells and bio-engineering cells muted by outer space is established. ②No distinct chromosome abnormality is found among bio-engineering cells muted by outer space. The abnormal chromosome rate of tumor cells muted by outer space is increased.

关 键 词：卫星搭载 肿瘤细胞 生物工程细胞 染色体制备 核型分析 

分 类 号：R73-3[医药卫生—肿瘤]