骨肉瘤、骨巨细胞瘤P53基因蛋白表达的定量研究  

QuantitativeStudyofP53GeneProteinExpressioninOsteosarcomaandGiantCelTumorofBoneDongyuchang

作　　者：董玉昌[1,2] 左连富[1,2] 丁文元[1,2] 张标[1,2] 高石军[1,2] 

机构地区：[1]河北医学院附三院骨病科 [2]河北省肿瘤研究所

出　　处：《中华骨科杂志》1996年第5期324-326,共3页Chinese Journal of Orthopaedics

摘　　要：作者应用流式细胞测量及免疫荧光技术对骨肉瘤和骨巨细胞瘤细胞Ｐ５３基因蛋白表达进行了定量研究，探讨了Ｐ５３蛋白表达与ＤＮＡ倍体、细胞增殖活性和病理学及预后的关系。结果显示，骨肉瘤和Ⅲ级骨巨细胞瘤均为ＤＮＡ异倍体，Ｐ５３蛋白均为高表达，ＤＮＡ含量（ＤＩ值）、ＰＩ值和Ｐ５３蛋白表达量（ＦＩ值）明显高于Ⅰ、Ⅱ级骨巨细胞瘤，并与骨巨细胞瘤组织学分级密切相关。Ｐ５３蛋白的表达量与骨肉瘤。Introduction:P53geneproteinexpressioninosteosarcomaandgiantceltumorofbonewerequantitativelystudiedusingflowcytometryandimmunofluorescencestainingtechnique,andasso-ciatedwithDNAploidy,Celularproliferationactivity,pathologyandprognosis.MaterialandMeth-ods:Thetissueblocksofparaffin-embeddedmaterialwastakenfrompatientswith15casesosteosarco-ma,25casesgradedgiantceltumorofboneand3casesbenigncysticofboneforcontrolsample.ThecelularDNAwasstainedwithethiumbromide.TheinterimmunofluorescencestainingwasperformedwithP53monoclonalantibody(PAb1801).DNAcontentandP53proteinexpressionwereanalysedquantitativelyusingFAcs420flowcytometer.Results:TheresultsshowedthataltheosteosarcomaandⅢgradegiantceltumorwereDNAaneuploidandP53proteinhighexpression.DNAcontent(DI),PIandP53expressioncontent(FI)inosteosarcomaandⅢgradegiantceltumorofbonewerehigherthanthatinⅠⅡgradegiantceltumorofbone.DI,PIandFIvalueswereassociatedwithhis-tologicalgradeingiantceltumorofbone.P53proteinexpressioncontentwasrelatedtoprognosisinosteosarcomaandgiantceltumorofbone.Conclusion:P53proteinexpressionwasanalysedquantita-tivelyforanewmarkerofprognosiswithmalignanttumorofbone.P53expressionpositivewasavalu-ablebiologicmolecularmessenger.

关 键 词：骨肉瘤 巨细胞瘤 P53 基因 流式细胞 骨肿瘤 

分 类 号：R738.102[医药卫生—肿瘤]