用ELISA法快速检测上皮性卵巢癌的研究  

Rapid detection of epithdian ovary cancer by ELISA

作　　者：朱丹[1] 卞美璐[1] 刘文淑[1] 许秀英[1] 

机构地区：[1]北京协和医院妇产科,北京中日友好医院妇产科

出　　处：《白求恩医科大学学报》1996年第2期157-159,共3页Journal of Norman Bethune University of Medical Science

摘　　要：本实验对１７７例上皮性卵巢癌患者抽取血清标本共２５１例次，以双抗体夹心法用酶联免疫吸附试验（ＥＬＩＳＡ）进行了检测，并与血清卵巢癌抗原１２５（ＣＡ１２５）放免测定进行了比较。结果ＥＬＩＳＡ法测定值超过２００ｍｇ／Ｌ为阳性；在良性卵巢瘤组中占１４．３％，其特异性为８５．７％；在上皮性卵巢癌术前患者中敏感性达８４％；对术后临床随访没复发的患者特异性为８９．９％；对术后临床随访有复发的患者敏感性为８２．５％；而与ＣＡ＿（１２５）放免测定结合其敏感性及特异性均有提高；可共同作为监测卵巢癌复发的指标。In this paper,we detected 251 serum samples derived from 177 patients with epithe-lian ovary cancer by using both ELISA(sandwich)and RIA of serum antigen of ovary cancer(CA125),respectively and made a comparison between these two assavs.All the results showed thatthe positive rate was 14.3%and the specificity was 85.7%with the assay of ELISA in the non-ma-lignant ovary tumour group.In addition,the sensitivity of pre-operation patients with epithelian ovarycancer was 84%. Furthermore,during the clinical follow-up,we also observed that the specificity ofthe post-operation patients without recurrence was 89.9%and the sensitivity of the post-operation pa-tients with recurrence was 82.5%,Combined application of the two assays could indeed increase thespecificity and the sensitivity in some extent.Thus,the two methods could be used as the parametersin monitoring the recurrence of ovary cancer.

关 键 词：上皮性 卵巢肿瘤 ELISA 

分 类 号：R737.310.4[医药卫生—肿瘤]