双歧杆菌脂磷壁酸对结肠癌细胞Toll样受体表达的影响  

The effect of lipoteichoic acid of Bifidobacterium on Toll-like receptor expression in colon cancer cells

作　　者：王跃[1] 罗强[1] 陈淑惠[1] 刘明方[1] 

机构地区：[1]重庆医科大学临床微生物学教研室,400016

出　　处：《中华微生物学和免疫学杂志》2005年第10期803-805,共3页Chinese Journal of Microbiology and Immunology

基　　金：重庆市教委科研项目(040308)

摘　　要：目的通过研究双歧杆菌脂磷壁酸(lipoteichoic acid,LTA)对结肠癌细胞中Toll样受体(TLR)表达的影响,探讨TLR在启动双歧杆菌LTA抗肿瘤信号转导通路中的作用。方法用RT-PCR检测结肠癌细胞HCT-116经双歧杆菌LTA处理前后TLR的表达变化。结果结肠癌HCT-116细胞有TLR的基础表达,且经LTA处理后受体的表达增强,呈剂量依赖性。结论双歧杆菌LTA可上调TLR的表达,提示双歧杆菌LTA诱导肿瘤细胞凋亡的信号转导途径可能由TLR启动。Objective To explore the signal transduction pathway of lipoteichoic acid （LTA） of Bifutobacterium in anti-tumor mechanism through investigating the effect of LTA on the expression of Toll-like receptor（TLR） in colon cancer cells. Methods Changes of TLR expression in colon cancer cells HCT-116 treated with LTA was detected by RT-PCR. Results The basic expression of TLR in HCT-116 cells was confirmed, and after treatment with different dose of LTA of Bifutobacterium, the expressions of TLR（ especially TLR2） were markedly increased in a dose-dependent manner. Conclusion LTA of Bifidobacterium can upregulate the expression of TLR in HCT-116 cells, it seems that TLR initiates the anti-tumor signal transduction of LTA of Bifidobacterium on apoptosis of colon tumor cells.

关 键 词：脂磷壁酸 双歧杆菌 TOLL样受体 表达 

分 类 号：R735.35[医药卫生—肿瘤]