转化生长因子β1对人牙髓细胞微丝骨架的重组作用  

TGF-β1 induced actin cytoskeletal reorganization in human dental pulp cells

作　　者：王凤明[1] 胡涛[1] 周学东[1] 

机构地区：[1]成都四川大学口腔生物医学工程教育部重点实验室,610041

出　　处：《实用口腔医学杂志》2005年第6期793-796,共4页Journal of Practical Stomatology

基　　金：四川省科技厅重点科技攻关项目(03SG022-002)

摘　　要：目的:了解转化生长因子β1(TGF-β1)对人牙髓细胞微丝和微管骨架的作用。方法:I型胶原酶消化组织块法体外培养人牙髓细胞,以20ng/ml的TGF-β1处理细胞,分别在第30min、1、6和24h收集细胞爬片,BODYPY-Phalloidin对微丝作直接荧光染色、Rhodamine RedTM对微管蛋白α(tubulin-α)作间接免疫荧光染色,采用激光扫描共聚焦显微镜观察TGF-β1作用于牙髓细胞后不同时间点微丝和微管的变化情况。结果:TGF-β1作用于人牙髓细胞后,微丝出现解聚重组现象,在30min时间点,肌动蛋白(actin)在细胞膜下聚合成纤维形肌动蛋白F-actin,同时胞质内的F-actin解聚,6h解聚最明显,24h后可见胞质内微丝重组。观察的各时间点,微管结构未见明显解聚重组现象。结论:TGF-β1能够使牙髓细胞微丝骨架重组。Objective ：To determine the effects of TGF-β1 on the cytoskeleton in cultured human dental pulp cells （HPCs）. Methods：Human dental pulp cells were cultured from dental pulp tissue explants digested with collagenase 1. Senti-confluent cultures of the cells maintained under serum deprivation were treated with 20 ng/ml of TGF-β1 for 30 min, 1 , 6 and 24 h respectively. Then cells were processed for BODYPY-phalloidin direct fluorescence examination of the actin filaments, DAP1 direct fluorescence of the nucleus and Rhodamine Red^TM indirect immuno-fluorescence of tubulin-α. Confocal laser scanning microscopy was used to investigate the changes of actin filaments and lnierotubules. Results：Disintegration and reorganization of actin filaments were observed in human dental pulp cells treated by TGF-β1 at 20 ng/ml. Actin filaments assembly was found near the cell membrane,specially after 30 min exposure. Disintegration of actin filaments was most obvious after 6 h treatment. Actin filaments were reorganized after 24 h exposure. Microtubules mainly remained intact in the cells during TGF-β1 treatment. Conclusion：TGF-β1 at 20 ng/ml may induce actin cytoskeletal reorganization in human dental pulp cells.

关 键 词：牙髓细胞 转化生长因子Β1 微丝 微管 

分 类 号：R78[医药卫生—口腔医学]