川芎嗪对兔肺缺血/再灌注损伤时血红素氧合酶-1表达与活性的影响  

EFFECT OF LIGUSTRAZINI ON THE EXPRESSION AND ACTIVITY OF HEME OXYGENASE-1 IN THE PULMONARY ISCHEMIA/REPERFUSION INJURY IN RABBITS

作　　者：王万铁[1] 周伟斌[1] 倪世蓉[1] 徐正祄[1] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325027

出　　处：《中国应用生理学杂志》2005年第4期427-431,共5页Chinese Journal of Applied Physiology

基　　金：浙江省跨世纪学术和技术带头人培养基金(992086);温州市"551人才工程"培养基金(98113);浙江省教育厅科研基金(20000670)

摘　　要：目的:探讨川芎嗪注射液对兔肺缺血/再灌注损伤时血红素氧合酶-1(HO-1)表达与活性的影响。方法:采用在体兔单肺原位缺血/再灌注损伤模型。实验兔20只,随机均分为肺缺血-再灌注损伤组(模型组)和川芎嗪注射液治疗组(川芎嗪组)。用免疫组化、原位杂交方法观察HO-1在兔肺组织中的表达变化;测定肺组织湿干重比(W/D)及肺泡损伤数(IAR);电镜观察肺组织超微结构的改变。结果:HO-1在模型组、川芎嗪组肺血管内皮、部分血管平滑肌、外膜层及部分气道上皮均有阳性表达,免疫组化(原位杂交)的平均吸光度值分别为0.168±0.016(0.148±0.013)、0.186±0.014(0.158±0.012)。川芎嗪组HO-1的表达水平明显高于模型组(P<0.01),W/DI、AR值显著低于模型组(P<0.01),肺组织形态学异常改变轻于模型组。结论:川芎嗪注射液可通过提高肺组织HO-1的表达水平,对肺缺血/再灌注损伤发挥积极的保护作用。Aim： To explore the effect of ligustrazini injection on the expression of heme oxygenase-1 （HO-1 ） in rabbits with pulmonary ischemia/reperfusion injury after. Methods： Single lung ischemia/reperfusion injury animal model was used in vivo. Twenty rabbits were randomly divided into two groups（ n 10,in each）, pulmonary ischemia/reperfusion injury（I/R） group and I/R ＋ ligustrazini injection （LGT） group. The tissue slides were stained by immunohistochemistry（IHC） and in situ hybridization（ISH） for HO- 1 to detect the expression of HO-1 in lung and to analyze the absorbance, wet to dry ratio of lung tissue weight（W/D） and the injured alveoli rate（lAIR） were measured at 180 minutes after lung reperfusion. Meanwhile the lung tissue slide was prepared for electron microscopic observation at 180 minutes after reperfusion. Results： HO-1 expression was upregulated in two groups in the pulmonary endothelial cells, part of pulmonary vascular smooth muscle cells, extima of vessels and epithelial cells of airway, the absorbance was 0. 168 ± 0. 016（0. 148 ± 0. 013）, 0. 186 ± 0. 014（0. 158 ± 0. 012 ）respectively. The LGTI group showed higher absorbance than those of the I/R group（P〈 0.01 ）,lower W/D and IAR values than those of the I/R group（P〈 0.01 ） significantly and lighter abnormal changes of the lung tissue in morphology than those of the I/R group. Conclusion： Ligustrazini injection possesses notable protective effects on I/R in rabbits by increasing the expression of HO-1 in lung.

关 键 词：缺A/再灌注损伤 肺 血红素氧合酶-1 川芎嗪注射液 

分 类 号：R363.2[医药卫生—病理学]