山茛菪碱对家兔全脑缺血再灌注后脑线粒体损伤的保护作用(英文)  

Protective effects of anisodamine on brain mitochondrial damage after complete cerebral ischemia and reperfusion in rabbits

作　　者：周代星[1] 钟强[1] 李政[2] 邓普珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北省武汉市430030 [2]华中科技大学同济医学院生化教研室,湖北省武汉市430030

出　　处：《中国临床康复》2005年第41期165-167,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:山莨菪碱是从茄科唐古特莨菪中提取的一种生物碱,是一种良好的细胞保护剂。而线粒体功能变化是反映细胞损伤的最敏感指标之一。目的:观察山莨菪碱对家兔全脑缺血再灌流后脑线粒体损伤的影响,探讨山莨菪碱的脑保护作用。设计:完全随机对照实验。单位:华中科技大学同济医学院附属同济医院。材料:实验于2002-09／12在华中科技大学同济医学院附属同济医院急诊科实验室完成。选择30只健康家兔,随机分为假手术组、缺血再灌流组、山莨菪碱组,每组10只。方法:采用“结扎双侧椎动脉和夹闭双侧颈总动脉+体循环低血压”建立全脑缺血再灌流模型,缺血20 min,再灌流2 h。山莨菪碱组于再灌流前 1 min由股静脉给予山莨菪碱,剂量为10 mg／kg,并以5 mg／h速度维持治疗2 h。缺血再灌流组再灌流期间给予等量生理盐水治疗;假手术组只接受手术,但不结扎和夹闭血管。①采用氧化电极法测定脑线粒体呼吸功能,包括呼吸控制率,磷氧比,氧化磷酸化效率。②采用氧电极法测定脑线粒体内还原型烟酰胺腺瞟呤二核苷酸氧化酶,琥珀酸氧化酶,细胞色素C氧化酶活性。③采用原子吸收光谱法测定脑线粒体钙含量。④采用改良八木国夫法测定脑线粒体丙二醛含量。主要观察指标:①各组家兔大脑皮质线粒体呼吸功能、呼吸链氧化酶活性变化。②各组家兔大脑皮质线粒体内钙和丙二醛含量。结果:30只家兔均进入结果分析。①各组家兔大脑皮质线粒体呼吸控制率、磷氧比、氧化磷酸化效率:缺血再灌流组和山莨菪碱组低于假手术组[烟酰胺腺嘌呤二核苷酸链:呼吸控制率:2．34±0．18,3．58±0．29, 4．07±0.38,P<0．05-0．01;磷氧比:1．77±0．10,2．23±0．14,2．41±0．17, P<0．05-0．01;氧化磷酸化效率:(5．27±0．78),(8．03±1.30),(9．63±150)μkat／g, P<0.05-0．01;黄素腺嘌呤二核苷酸链:呼吸BACKGROUND： Anisodamine, a kind of alkaloid, is extracted from Anisodus tanguticus （Maxim.） Pascher and is also a good protective agent of cell. However, functional change of mitochondrion is the most sensitive index reflecting cell injury. OBJECTIVE： To study the effects of anisodamine on brain mitochondrial damage following global cerebral ischemia and reperfusion in domestic rabbits and explore its mechanism. DESIGN： Totally randomized controlled trials. SETTING： Emergency Department of Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology. MATERIALS： The experiment was carried out in the laboratory of Emergency Department, Tongii Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, from September to December in 2002. Thirty healthy domestic rabbits of either sex were used and randomized into sham-operation group, ischemia-reperfusion group and anisodamine group with 10 rabbits in each group. METHODS： The models of complete cerebral ischemia and reperfusion injury in rabbits were established by ligation of bilateral common carotids and vertebral arteries with systemic hypotension, ischemia lasting for 20 minutes followed by 2-hour reperfusion. Anisodamine group was injected with anisodamine at a dose of 10 mg/kg body mass via femoral vein one minute before reperfusion, and lasted for 2 hours at a dose of 5 mg/hour by micro-pump, lschemia-reperfusion group was treated with normal saline of the same volume. Sham-operation group only underwent operation without vessel occlusion. ① The oxygen-electrode method was used to determine mitochondrial respiratory functions, including respiratory control rate （RCR）, the ratio of adenosine diphosphate to oxygen （ADP/O） and oxidation-phosphoration rate （OPR）. ② The activities of nicotinamide adenine dinucleotide hydrogenated （NADH） oxidase, succinate oxidase and cytochrome C oxidase were measured by the oxygenelectrode method. ③Mitochondrial Ca^2＋ was measur

关 键 词：脑缺血 再灌流 山莨菪碱 线粒体 

分 类 号：R743.31[医药卫生—神经病学与精神病学]