灯盏花素对缺血再灌注鼠脑海马三磷酸腺苷含量及其活性变化的干预(英文)  

Effects of erigeron on hippocampus adenosine triphosphate of gerbils with cerebral ischemia reperfusion injury

作　　者：张焰[1] 陈群[2] 丁浩中[1] 曾因明[2] 

机构地区：[1]苏州大学附属第四医院麻醉科 [2]徐州医学院附属医院麻醉科,江苏省徐州市221002

出　　处：《中国临床康复》2005年第41期176-178,F0003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:灯盏花素作为蛋白激酶C的抑制剂,具有降低脑缺血再灌注引起的神经细胞死亡作用。目的:探讨灯盏花素对缺血再灌注沙土鼠脑海马中三磷酸腺苷的含量, 以及对三磷酸腺苷酶活性的影响。设计:随机对照区组设计。单位:苏州大学附属四院麻醉科,无锡市第四人民医院麻醉科,徐州医学院附属医院麻醉科。材料:实验于2002-03／2003-05在江苏省麻醉学重点实验室完成。选取蒙古沙土鼠90只,随机分为9组:假手术组、脑缺血组、脑缺血再灌注1 h组、脑缺血再灌注12 h组、脑缺血再灌注24 h组、灯盏花素+脑缺血组、灯盏花素+脑缺血再灌注1 h组、灯盏花素+脑缺血再灌注12 h 组、灯盏花素+脑缺血再灌注24 h组,10只／组。灯盏花素注射液(云南省生物制药厂生产,批号990103,每毫升含黄酮4．5 mg)。方法:除假手术组外,其余各组均建立缺血再灌注模型。灯盏花素各组在缺血前30 min腹腔注射灯盏花素10 mL／kg,其余组同期注射等量生理盐水。每组抽取6只行三磷酸腺苷含量及三磷酸腺苷酶活性测定,其余4只进行海马CA1区神经细胞病理学检查。在缺血期间监测鼓膜温度(反映脑温),并维持鼓膜温度于(37+0．2)℃,以避免温度变化对实验结果的影响。组间比较采用单因素方差分析。主要观察指标:①各组实验鼠脑海马三磷酸腺苷含量和三磷酸腺苷酶活性的变化。②各组实验鼠脑海马CA1区神经细胞病理学变化。结果:实验纳入蒙古沙土鼠90只,全部进入结果分析。①各组海马三磷酸腺苷含量和三磷酸腺苷酶活性的变化:与脑缺血再灌注24 h组比较,灯盏花素1,12,24 h组三磷酸腺苷含量均明显提高[(0．25±0．08),(0．81 ±0．12),(0．58±0．07),(0．43±0．09)mmol／kg,P<0．01],Na+-K+ATP酶活性均明显上升[(1．23±0．43),(5．09±0.36),(3．67±0．37),(2．71±0．42)mmoL／(g·h), P<0.01],Ca2+-ATP酶活性亦明显上升[(0.58±0.35BACKGROUND： Erigeron, the inhibitor of protein kinase C, functions to decrease the death of neurocytes caused by cerebral ischemia reperfusion. OBJECTIVE： To study the effects of erigeron on hippocampus adenosine triphosphate （ATP） and adenosine triphosphatase （ATPase） of gerbils with cerebral ischemia reperfusion injury. DESIGN： A random controlled experiment. SETTING： Anesthesia Department of the Fourth Hospital of Suzhou University, Anesthesia Department of the Fourth People＇s Hospital of Wuxi City, and Anesthesia Department of the Affiliated Hospital of Xuzhou Medical College. MATERIALS： The experiment was done in the key laboratory of anesthesiology of Jiangsu Province from March 2002 to May 2003. The 90 Mongolian gerbils were divided into 9 groups： sham operated group, cerebral ischemia group, cerebral ischemia reperfusion 1 hour group, cerebral ischemia reperfusion 12 hours group, cerebral ischemia reperfusion 24 hours group, erigeron ＋ cerebral ischemia group, erigeron ＋ cerebral ischemia reperfusion 1 hour group, erigeron ＋ cerebral ischemia reperfusion 12 hours group, and erigeron ＋ cerebral ischemia reperfusion 24 hours group, with 10 gerbils in each. The erigeron injection with flavone 4.5 g/mL was made by the Biomedicine Factory of Yunnan Province and numbered 990103. METHODS： Apart from the sham operated group, all groups were established the ischemia reperfusion models. The erigeron groups were abdominally injected erigeron 10 mL/kg 30 minutes before the ischemia. Meanwhile, the other groups were injected the normal saline 10 mL/kg. The 6 gerbils of each group were taken blood for testing ATP content and ATPase activity and the rest 4 were only examined the pathological changes of neurocytes of hippocampus CA1 region. During the ischemia, the temperature of drum membrane （reflecting the brain temperature） was monitored and kept at （37±0.2）℃ in order to avoid the influence of temperature on the result. The comparison among groups was shown with the single f

关 键 词：再灌注损伤 腺苷三磷酸 腺苷三磷酸酶 灯盏细辛 蛋白激酶C 

分 类 号：R743.31[医药卫生—神经病学与精神病学]