经兔肝动脉灌注平阳霉素碘油乳剂制作肝纤维化模型  

A new model of hepatic fibrosis induced by intra-arterial injection of iodized oil and pingyangmycin emulsions

作　　者：李枫 徐克[1] 刘瑞宝[1] 

机构地区：[1]中国医科大学附属第一医院放射科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2005年第17期2090-2093,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨经兔肝动脉灌注平阳霉素碘油乳剂(PLE)制作肝纤维化模型的可行性.方法:4-5月龄日本大耳白兔24只,按经肝动脉注入PLE量的不同分成假手术组,实验A组、实验B组,分别于术后1、2、4、6、10、14wk定期开腹取材,做HE染色及血小板衍化生长因子B(PDGF-B)免疫组化染色.结果:6wk时A组及B组兔肝脏均可见明显纤维化改变,部分肝组织假小叶形成.B组兔10wk时4只兔中有一只出现一直径约1.0cm大小的硬化结节,光镜下为团状增生的纤维组织.PDGF-B免疫组化染色可见肝细胞胞膜及纤维间隔中大量阳性染色.A、B组积分光密度值在注射后1、2、4、6wk比假手术组明显升高(118714±14941,154344±25102vs42745±871,P<0.01;130399±11690,164855±15486vs44052±1043,P<0.01;116594±21230,147099±20317vs41081±769,P<0.01;101732±8794,124177±20429vs46366±975,P<0.01).结论:经肝动脉灌注PLE制作肝纤维化模型成功率高,死亡率低.PDGF-B作为肝纤维化的启动因子参与了该模型的纤维化过程.AIM： To investigate the feasibility of setting up a hepatic fibrosis model by intra-arterial injection of emulsions of iodized oil and pingyangmycin. METHODS： Twenty-four Japanese big-ear rabbits were divided into sham-operation, experiment A and B group according to the doses of emulsions of iodized oil and pingyangmycin （1.2 and 2.0 mg for A and B, respectively） injected into the hepatic artery. The live specimens were obtained 1, 2, 4, 6, 10, and 14 wk after injection. The histological changes were determined by HE staining, and the contents of platelet derived growth factor-B （PDGF-B） were detected by immunohistochemical staining. RESULTS： Six weeks after injection, typical features of fibrosis appeared in all the liver tissues of group A and B, and the pseudolobules formed in some tissues. In group B, sclerosis with a diameter of 1.0 cm was found in one of the four rabbits at 10th week under light microscope. The contents of PDGF-B were significantly increased in A and B group as compared with those in sham-operation group 1, 2, 4, and 6 wk after injection （integral optical density：118714±14941,154344±25102 vs42745±871,P〈0.01;130399±11690,164855±15486 vs44052±1043, P 〈0.01;116594±2.1230,147099±2.0317 vs41081±769, P 〈0.01;101732±8794,124177±20429 vs 46366±975, P 〈0.01）. CONCLUSION： The model of hepatic fibrosis can be established successfully by injection of iodized oil and pingyangmycin emulsions into the hepatic artery. PDGF-B plays an important role in the progress of fibrosis as a promoter.

关 键 词：平阳霉素 碘油 肝纤维化 模型 

分 类 号：R-332[医药卫生]