胃癌组织环氧合酶-2表达与幽门螺杆菌cagA^+菌株感染  

Relationship between expression of cyclooxygenase-2 and cagA^+ Helicobacter pylori infection in gastric carcinoma

作　　者：张勇[1] 蒋明德[1] 曾维政[1] 徐辉[1] 王钊[1] 陈虹彬[1] 

机构地区：[1]中国人民解放军成都军区总医院消化内科,四川省成都市610083

出　　处：《世界华人消化杂志》2005年第17期2141-2144,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨幽门螺杆菌(Helicobacterpylori,Hpylori)cagA菌株感染及环氧合酶-2(cyclooxygenase-2,COX2)表达在胃癌发生中的作用及两者的关系.方法:采用原位聚合酶链反应(PCR)技术及免疫组织化学技术检测56例胃癌手术标本cagA基因及COX2蛋白表达情况.结果:胃癌组织HpyloricagA菌株感染率为55.4%,COX2在胃癌组织中表达率为71.4%.COX2表达水平在Ⅲ-Ⅳ期胃癌组显著高于Ⅰ-Ⅱ期胃癌组(14/30vs5/26,P<0.05);有淋巴结转移组明显高于无淋巴结转移组(4/19vs15/37,P<0.01);HpyloricagA菌株感染与TNM分期及淋巴结转移无关(P>0.05).COX2表达水平在HpyloricagA阳性组显著高于HpyloricagA阴性组(15/31vs4/25,P<0.05).结论:HpyloricagA菌株感染与胃癌的恶性进展无关;COX2的表达参与胃癌的发生及恶性进展.HpyloricagA菌株感染能上调胃癌组织COX2的表达.AIM： To explore the relations of cagA^＋ Helicobacter pylori infection with the expression of cyclooxygenase-2 （COX2） in human gastric carcinogenesis. METHODS： In situ polymerase chain reaction （PCR） was used to detect the expression of Hpy/ori cagA gene, and immunohistochemical technique was used to detect the expression of COX2 in 56 patients with gastric carcinoma. RESULTS： The rates of cagA^＋ Hpy/ori infection and COX2 expression were 55.4% and 71.4%, respectively, in gastric carcinoma. The level of COX2 expression was significantly higher in the gastric carcinoma of Ⅱ- Ⅳ stage than Ⅰ -Ⅱ stage （14/30 vs 5/26, P 〈0.05）, and was also higher in gastric carcinoma with lymph node metastasis than that without lymph node metastasis （4/19 vs 15/37, P 〈0.01）. CagA^＋ Flpylori infection was not correlated with TNM stage and lymph node metastasis in gastric carcinoma （P 〉0.05）. The level of COX2 expression was significantly higher in cagA^＋ Flpylorigroup than that in cagA Flpylori group （15/31 vs 4/25, P 〈0.05）. CONCLUSION： CagA^＋ H pylori infection is not related with the malignant progression of gastric carcinoma, but COX2 expression is involved in the carcinogenesis and malignant progression of gastric carcinoma. CagA^＋ H pylori infection up-regulates the expression of COX2 in gastric cancer.

关 键 词：CAGA^+ H pylori感染 环氧合酶-2 原位聚合酶链反应 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]