脂多糖结合蛋白(LBP)基因多态性对LBP及细胞因子诱生的影响  

Effect of lipopolysaccharide binding protein gene polymorphism on lipopolysaccharide-induced LBP and inflammatory cytokine levels

作　　者：蔺静[1] 姚咏明[1] 咸力明 黄志红[1] 侯晓霞 于燕[1] 

机构地区：[1]解放军总医院第304临床部全军烧伤研究所基础部,北京100037

出　　处：《中华实验外科杂志》2005年第12期1425-1426,共2页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展规划项目(G1999054203;2005CB522602)国家杰出青年科学基金资助项目(30125020)北京市科技计划重大项目(H020920020530)军队十五医药卫生科研基金资助项目(01MA207)

摘　　要：目的探讨脂多糖结合蛋白(LBP)C1306→T单核苷酸多态性(SNP)对全血培养LBP 表达及细胞因子生成的影响。方法采集118例健康献血员静脉血,运用聚合酶链反应(PCR)及限制性内切酶Stu I对PCR产物的消化作用检测C1306→T基因多态性,并采用全血细胞培养模型检测内毒素刺激前后LBP、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6及IL-10蛋白水平。结果 118例健康献血员中,14例为T／C杂合子,104例为T／T纯合子。对照组及内毒素刺激组中T／C 基因型上清液LBP浓度均明显高于T／T纯合子,而TNF-α、IL-6及IL-10对内毒素的反应性在两种基因型之间差异不明显(P>0．05)。结论内毒素受体LBP C1306→T单核苷酸多态性可能间接影响LBP的蛋白水平,但与体外炎症介质的生成关系不明显。Objective 1 o evaluate whether the hpopolysaccharide binding protein （LBP） C1306→T single nucleotide polymorphism influences the LBP and inflammatory cytokine levels in whole blood culture. Methods In 118 healthy human blood donors, the Cl306→T gene polymorphism was determined by using polyrnerase chain reaction and subsequent Stu I restriction enzyme digestion of the polyrnerase chain reaction products, Supernatant concentrations of LBP, TNF-α IL-6 and IL-10 were measured by using a whole blood cell culture model in the presence or absence of lipopolysaccharide （LPS） stimulation. Results Among the 118 individuals, 14 subjects were heterozygous and 104 homozygous for the T/T allele. The LBP levels in supernatant were higher in T/C heterozygotes than in the individuals homozygous fog the T allele either before or after LPS stimulation, while no marked correlations were found between LBP C1306→T polymorphism and TNF-α IL-6 or IL- 10 formation to LPS （ P 〉 0.05）. Conclusion The LBP Cl306→T polymorphism may influence the LBP level indirectly, but did not significantly relate to inflammatory cytokine in vitro.

关 键 词：脂多糖结合 单核苷酸多态性 内毒素 基因多态性 细胞因子 

分 类 号：R362[医药卫生—病理学]