免疫隔离技术在异种肝细胞移植中的应用  

Microencapsulated porcine hepatocyte transplantation to treat acute hepatic failure of rats induced by drug

作　　者：刘菲[1] 刘雁冰[1] 熊伍军[1] 钟岚[1] 赵中辛[2] 

机构地区：[1]同济大学附属东方医院消化科,上海200120 [2]同济大学附属东方医院普外科,上海200120

出　　处：《中华实验外科杂志》2005年第12期1450-1451,i0020,共3页Chinese Journal of Experimental Surgery

基　　金：上海市浦东新区科学技术局基金资助项目(PK2001-13)

摘　　要：目的探讨微囊化猪肝细胞腹腔内移植对药物性肝衰大鼠的治疗作用,观察移植大鼠存活率、肝功能的变化。方法以海藻酸钠体外包裹经胶原酶灌注法分离的乳猪肝细胞,以SD 大鼠为受体,D-氨基半乳糖腹腔内注射诱导大鼠急性肝衰竭,48 h后将微囊化的猪肝细胞移植于大鼠腹腔内,观察移植大鼠存活率、绘制生存曲线,并测定肝功能的变化。结果腹腔内肝细胞移植可显著改善大鼠肝功能,转氨酶及胆红素均较对照组明显下降(P<0．05)。与裸肝细胞移植组相比,微囊化肝细胞移植组1周存活率(78．6％vs 66．7％)及2周存活率(42．9％vs 25．0％)均显著提高(P<0．01)。结论对异种肝细胞经微囊化处理后移植治疗急性肝衰大鼠,可给予肝功能代谢支持,提高移植治疗效果。Objective To investigate the therapeutic effect of microencapsulated porcine hepatocytes transplanted intraperitoneally on toxic hepatic failure in rats and observe the survival rate and the change of hepatic function of the recipient rats. Methods The collagenase perfusing method was used to separate hepatocytes from pup pig liver. And then the hepatocytes were encapsulated by the alginate method and transplanted into abdominal cavity of D gal-induced acute hepatic failure （AHF） rats. The survival rate and hepatic function in the rats were determined. Results Hepatocytes transplanted intraperitoneally could improve greatly hepatic function of rats, and the ALT, AST and TB were all improved significantly （P〈 0.05 ） as compared with NS injection group. The 1- and 2-week survival rate in mieroencapsulated porcine hepatocyte transplantation group was longer than in free hepatocyte transplantation group （78.6% vs66.7%,42.9% vs25.0% respevtively, P〈0.01）.Conclusion Microencapsulated xenogeneic hepatocyte transplantation is effective in the treatment of AHF.

关 键 词：肝细胞 微囊化 肝衰竭 细胞移植 免疫隔离技术 

分 类 号：R657.3[医药卫生—外科学] R329.2[医药卫生—临床医学]