p14ARF蛋白在人肝细胞癌中的表达缺失及其临床意义  

The deletion of p14ARF protein in human hepatic carcinoma and its significance

作　　者：包仕廷[1] 王三明[1] 林木生[1] 缪辉来[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东湛江524001

出　　处：《中华实验外科杂志》2005年第12期1455-1456,共2页Chinese Journal of Experimental Surgery

摘　　要：目的研究人肝细胞癌中p14ARF蛋白的表达缺失情况及其与临床病理的关系。方法采用链霉菌素-生物素(SP)免疫组织化学法及免疫印记反应(Western blot)对45例肝细胞癌组织、39例癌旁组织及37例非肿瘤肝组织标本进行检测分析。结果 p14ARF蛋白在肝癌肿瘤组织中表达缺失率为84．4％(38／45),p14ARF蛋白I级-Ⅱ级(高分化)病例p14ARF蛋白缺失率为 44．4％,Ⅲ级-Ⅳ级(低分化)病例p14ARF蛋白缺失率为94．4％(P<0．05)。结论 p14ARF蛋白表达缺失与肝细胞癌的发生、发展有一定相关性。提示 p14ARF基因的失活可能是肝细胞癌恶性演变的重要因素。Objective To investigate the deletion of P14ARF and its relation with clincial pathology. Methods The expression of P14ARF was detected in 45 cases of hepatic carcinoma, 39 paracancerous tissues and 37 non-tumor liver tissues by using streptavidin peraxidase （SP） immunohistoehemical assay and Western blot. Results The percentage of P14ARF deletion in hepatic carcinoma of grade Ⅰ-Ⅱ and those of grade Ⅲ-Ⅳ were 44.4% and 94.4%, respectively （P〈0.05）.Conclusion The deletion of P14ARF protein might be correlated with the pathologic grading of the hepatic carcinoma, suggesting the inactivation of P14ARF gene may be an important factor of carcinogenesis and development of hepatic carcinoma.

关 键 词：P14ARF蛋白 肝细胞癌 抑癌基因 表达缺失 

分 类 号：R735.7[医药卫生—肿瘤]