脑表面大体摄影评价小鼠局灶性脑缺血后24h内血脑屏障通透性变化  

Photomacrography of brain surface for evaluating blood-brain barrier disruption within 24 h after focal cerebral ischemia in mice

作　　者：陈丽萍[1] 徐慧敏[1] 赵炜[1] 张世红[1] 朱朝阳[1] 张琦[1] 余国良[1] 储利胜[1] 魏尔清[1] 

机构地区：[1]浙江大学医学院药理学教研室

出　　处：《浙江大学学报（医学版）》2005年第6期523-528,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(30271498)资助项目;浙江省自然科学基金(399090)资助项目

摘　　要：目的:建立脑表面大体摄影结合图像分析的方法,观察小鼠局灶性脑缺血后24 h内血脑屏障通透性的变化。方法:采用线栓法诱导小鼠局灶性脑缺血,于缺血后10、30 m in和1、3、6、12、24 h取脑,用数码相机拍摄全脑及脑切片照片,观察脑表面和切面的出血和伊文思蓝(EB)渗出,以及血脑屏障通透性变化;并用荧光法测定脑内EB含量,以干湿重法检测脑水分含量。结果:脑表面图像分析显示,缺血后3 h开始出现明显的出血和EB渗出,与脑切片的结果一致,具有较好的相关性;荧光法显示缺血后30 m in缺血侧脑组织EB含量开始增高,缺血后1 h脑水分含量开始增加。结论:脑表面大体摄影结合图像分析,可对局灶性脑缺血后血脑屏障通透性的变化进行半定量评价,还发现脑缺血后早期血脑屏障的通透性就有增加。Objective： To establish a new photomacrographic analysis of morphological changes on brain surface to evaluate blood-brain barrier （BBB） disruption. Methods： Permanent focal cerebral ischemia was induced by middle cerebral artery occlusion （MCAO） in mice. Brains were removed 10 min,0. 5,1,3,6, 12 and 24 h after MCAO. The whole brains and brain slices were photographed by a digital camera. BBB disruption was evaluated by hemorrhage and traced Evans blue （EB） on the brain surface. Fluoremetric quantitation of EB and water content in the brains were also performed at various time points. Results ： Photomacrographic morphological analysis showed that hemorrhage and traced El3 on the surface of the brains significantly increased from 3 h after focal cerebral ischemia, which were correlated to the results in the brain slices. EB content in the ischemic hemispheres was significantly increased from 0. 5 h after MCAO, and water content was increased from 1 h after MCAO. Conclusion： Photomacrographic measurement is a simple and useful method for evaluating BBB disruption semiquantitatively,and can detect BBB disruption earlier after focal cerebral ischemia in mice.

关 键 词：脑缺血 血脑屏障通透性 大脑中动脉阻塞 大体摄影法 

分 类 号：R743[医药卫生—神经病学与精神病学]