大肠癌患者手术期间门静脉血中癌细胞CK20mRNA的检测及意义  

Significant detection of circulating cancer cells in the portal vein blood during colorectal cancer resection

作　　者：周连锁[1] 陈惠玉[1] 唐强[1] 周爱明[1] 金灌培[1] 

机构地区：[1]江苏省连云港市第一人民医院肿瘤外科,连云港222002

出　　处：《陕西医学杂志》2005年第12期1464-1466,共3页Shaanxi Medical Journal

基　　金：广东省医学科学技术研究基金(A2001453);江苏省连云港市科委学科带头人基金(QN200202)

摘　　要：目的:研究手术刺激对大肠癌患者门静脉血中癌细胞的影响。方法:应用RT-PCR技术,检测82例大肠癌患者术前和术后门静脉血中CK20mRNA的表达。结果:手术前54.9%的患者CK20mRNA表达阳性,CK20mRNA阳性检出率与Dukes分期、淋巴结转移、肝转移存在差别有显著性。手术后门静脉血中有76.8%的患者CK20mRNA表达阳性,较术前阳性表达明显增多(P<0.005)。结论:门静脉血液中癌细胞的存在是大肠癌的一种常见的早期事件,手术刺激可增加肿瘤细胞向门静脉血中释放。Objective： To study the change of cancer cells in the portal vein blood during colorectal cancer resection. Methods： Cytokeratin 20 mRNA in the portal vein blood of 82 patients with colorectal carcinoma was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） during colorectal cancer resection. Results： The positive rate of CK20mRNA in portal vein blood was 54. 9%. The positive detection was significiantly correlated with Duckes stage, lymph node metastasis and liver metastasis. The positive rate of CK20mRNA in the portal vein blood was 76.8% after operation. It has singnfficently higher than before operation （P〈0. 005） . Conclusion, Molecular detection in the portal vein blood at surgery suggests that hematogenic tumor cell dissemination is a common and early event and that surgical manipulation enhances this release of tumor cells into the circulation.

关 键 词：大肠肿瘤 角蛋白 门静脉 外科手术 

分 类 号：R735.34[医药卫生—肿瘤] R575.205[医药卫生—临床医学]