支气管哮喘患儿与RANTES单核苷酸多态性的相关性研究  

The SNPs of chemokine RANTES promoter in children with asthma

作　　者：刘梅[1] 李海林[1] 黄永坤[1] 陈燕华[1] 刘华[2] 金萍[1] 

机构地区：[1]昆明医学院第一附属医院儿科,云南昆明650032 [2]昆明医学院第一附属医院检验科PCR实验室,云南昆明650032

出　　处：《中国优生与遗传杂志》2005年第11期20-23,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨RANTES单核苷酸多态性(SNP)与哮喘易感惠儿的相关性。方法 采用聚合酶链反应方法对32例哮 喘患儿和32例正常对照进行了RANTES-403及-28位点基因型分布和等位基因频率研究。结果 病例组RANTES-403位 点G/G、G/A、A/A基因型频率分别为53．13％、40．62％、6．25％;对照组分别为50．00％、43．75％、6．25％(P>0．05)。 两组突变等位基因-403A频率分别为26.53％和28．13％(P>0．05)。病例组RANTES-28位点C/C、C/G、G/G基因型频率分别为 78．13％、18．75％、3．12％;对照组分别为90．63％、9．37％、0％(P>0．05)。两组突变等位基因-28G频率分别为12．50％和 4．69％(P>0．05)。结论 RANTES-403位点和-28位点SNP与哮喘发病可能没有直接的关系。Objective： To detect the association between the single nucleofide polymorphism （SNPs）of chemokine RANTES in children with asthma. Methods：We use PCR- RFLP to study the SNPs of RANTES polymorphism at - 403 and - 28 sites in the blood specimens of 32 children with asthma and 32 controls. Results：The genotyping frequencies of RANTES - 403 G/G,G/A and A/A in case group were 53.13%, 40.62 % and 6.25 %, respectively; while the genotyping frequencies of RANTES - 403 G/G, G/A and A/A in control were 50,00 %, 43.75 % and 6.25%, respectively（ P 〉 0.05） .Them is no significantly difference in the frequency of mutation - 403A allele between case group and controls （26.53% vs 28.13%, P 〉 0.05）. The genotyping frequencies of RANTES - 28 C/C, C/G and G/G in case group were 78.13%, 18.75 % and 3.12%, respectively; which were not significantly different from those in controls as 90.63% ,9.37% and 0% （ P 〉 0,05）. There is no significantly difference in the frequency of mutation - 28G allele between asthma and controls （12.50 % vs 4.69 %, P 〉 0.05）. Conclusions：These results indicate that the SNPs of RANTES promoter are may not associated asthma.

关 键 词：等位基因 基因型 频率 RANTES多态性 哮喘 

分 类 号：R725.6[医药卫生—儿科]