慢性吗啡处理大鼠伏隔核、海马CA1区神经元超微结构改变  

Changes of ultrastructures of neurons in nucleus accumbens, hippocampal CA1 of chronic morphine treated rats

作　　者：张瑞岭[1] 叶敏杰[1] 郭新胜[1] 潘苗[1] 杜保国[1] 郝伟[2] 李毅[2] 徐锡萍[3] 

机构地区：[1]新乡医学院第二附属医院中心实验室,新乡453002 [2]中南大学湘雅二医院精神卫生研究所,长沙410011 [3]中南大学湘雅医学院电镜室,长沙410086

出　　处：《郑州大学学报（医学版）》2005年第6期1049-1051,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：美国中华医学基金会资助项目CMB96648;国家自然科学基金资助项目30370522;973项目子课题2003CB515403

摘　　要：目的:探讨慢性吗啡处理大鼠伏隔核及海马CA1区神经元超微结构改变。方法:将10只雄性SD大鼠随机等分为吗啡组(腹腔注射吗啡,起始剂量5mg/kg,2次/d,逐d递增5mg,至第10d为50mg/kg)及对照组(用相同方式注射同体积的生理盐水)。末次注射后6d取伏隔核及海马CA1区。制作电镜标本后在透射电镜下定性观察神经元超微结构。结果:吗啡组大鼠伏隔核神经元核膜欠清晰,部分线粒体结构模糊,部分内质网有轻度扩张,而对照组正常;吗啡组大鼠海马CA1区神经元部分核膜结构节段性模糊不清,部分线粒体结构模糊,嵴消失,甚至空泡变,而对照组正常。结论:慢性吗啡处理大鼠伏隔核、海马CA1区神经元产生了一定程度的超微结构病理改变。Aim： To explore the changes of uhrastructures of neurons in nucleus accumbens （ NAc）, hippocampal CA1 （ HIPCA1 ） of chronic morphlne-treated rats. Methods ： Ten male SD rats were randomly allocated into morphine group （intraperitoneally injected morphine twice a day for 10 d in ascending dosage schedule） or control group （injected normal saline of the same volume）. Rats were killed on the 6th d following the last injection. NAc and HIPCA1 were removed and prepared. The uhrastructures of neurons in NAc and HIPCAI, including membrane of nucleus, mitochondria, endoplasmic reticulum were investigated under transmission electron microscope. Results： Compared with control, the neurons in NAc of morphine-treated rats had the blurred nuclear membrane and mitochondria, and slightly dilated endoplasmic reticulum, while the neurons in HIPCA1 had the blurred membrane of nucleus and mitochondria, vanished matrix and even vacuole degeneration of mitochondria. Conclusion ： Chronic morphine treatment may cause the damage of ultrastructure of neurons in NAc and HIPCA1 of rats.

关 键 词：吗啡 伏隔核 海马CA1区 超微结构 大鼠 

分 类 号：R749.6[医药卫生—神经病学与精神病学]