HIF-1在早期糖尿病小鼠视网膜上的表达及作用  

Expression and role of the hypoxia inducible factors HIF-1 in the early diabetic mice retina

作　　者：李才锐[1] 胥延[2] 郭小健[1] 王平[1] 李雯霖[1] 洪卫[3] 姜德咏 

机构地区：[1]中南大学湘雅二医院眼科,长沙市410011 [2]昆明医学院二医院眼科,650101 [3]云南省大理医学院附院眼科,671000 [4]湖南博雅眼科医院,长沙市410000

出　　处：《实用医学杂志》2005年第23期2607-2609,共3页The Journal of Practical Medicine

基　　金：湖南省科技厅资助课题(编号:04SK3034/3)

摘　　要：目的:探讨糖尿病NOD小鼠视网膜HIF-1α表达及其作用。方法:NOD小鼠随机分为正常对照组和糖尿病组,分别于糖尿病2、4、6、8和12周处死糖尿病组和正常对照组小鼠,摘除眼球后分离视网膜。Westernblot方法检测视网膜HIF-1α的表达,免疫组化方法检测视网膜上HIF-1α的表达。结果:(1)与正常对照组相比,糖尿病组小鼠血糖明显增高,体重明显下降(P<0.01)。(2)Westernblot显示糖尿病6周时视网膜HIF-1α低水平表达,12周时HIF-1α表达增高。(3)免疫组化显示糖尿病组12周时视网膜内层HIF-1α表达增加,而未见视网膜外层HIF-1α表达。结论:(1)糖尿病早期视网膜HIF-1α表达增高,说明在糖尿病早期视网膜发生缺血缺氧。(2)HIF-1α可能参与了糖尿病早期视网膜缺血缺氧的继发损害过程。Objective To explore the levels of hypoxia inducible factors-1 （HIF-1） in retina of the diabetic NOD mice and its roles. Methods NOD mice were randomly divided into normal control group and diabetes group, and killed at 2, 4, 6, 8 and 12 weeks. Eyes were enucleated and retinas were isolated, and detected by Western blot, or eyes were sectioned for immunohistochemical staining for HIF-1α. Results ① Compared to the normal control group, blood glucose of diabetes mice increased significantly but the weights decreased significantly （P 〈 0. 01 ） . ②Western blot of retinal lysates showed low levels of HIF-1α at 6 weeks, which were markedly increased at 12 weeks in the diabetes group.③The HIF-1α staining in the inner retina showed increased, but not in the outer retina. Conclusion The retinal HIF-1α levels increasing shows that retinal ischemia happens in the early diabetes, and it may participate in the secondary damage of retinal isohemia induced by early diabetes.

关 键 词：糖尿病 视网膜 低氧诱导因子-1Α 

分 类 号：R774.1[医药卫生—眼科]