不同纳米颗粒二氧化钛的细胞相容性研究  

Study of cellular biocompatibility of different nano-size titania

作　　者：赵彦涛[1] 张玉梅[1] 宁芳[1] 许智轩[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《临床口腔医学杂志》2005年第12期732-734,共3页Journal of Clinical Stomatology

基　　金：西安市工业科技攻关计划项目(GG200356);第四军医大学后备人才基金资助

摘　　要：目的:探索二氧化钛发挥最佳生物学效应的纳米晶尺寸。方法:采用MTT法观察成骨细胞在材料表面的增殖情况,细胞培养第5d时对碱性磷酸酶活性进行检测,并用SEM观察第3d时细胞在材料表面的附着形态。结果:成骨细胞在20nm组材料表面的细胞增殖数显著高于其它各组,并具有更好的细胞伸展形态;而200nm组材料碱性磷酸酶表达最高。结论:不同纳米尺寸二氧化钛的细胞相容性存在差别。Objective：To explore the nano size of titania grains in which titania has the best cellular biocompatibility, Method：MTT method was used to evaluate the proliferation of osteoblast, ALP test was carried out on the fifth day, SEM was used to examin the morphology of osteoblast, Result： 20 nm titania has the most osteoblast cells, which spread better than cells on other group material, 200 nm group titania has the highest ALP level, Conclusion：Different nano-size titania had different cellular biocompatibility.

关 键 词：二氧化钛 纳米 细胞相容性 成骨细胞 

分 类 号：R780.2[医药卫生—口腔医学]