CIN和子宫颈鳞癌的HPV16/18感染与p53、MDM2蛋白表达的关系  

p53 and MDM2 expression with HPV16/18 infection in human uterine cervical carcinoma and cervical intra-epithelial neoplasia

作　　者：陈利友[1] 石一复[1] 周彩云[1] 张义龙[1] 陈晓端[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,浙江杭州310006

出　　处：《实用肿瘤杂志》2005年第6期500-503,共4页Journal of Practical Oncology

摘　　要：目的研究子宫颈鳞癌及C IN的HPV感染、p53蛋白和M DM 2蛋白的表达及三者之间的相互关系。方法选取子宫颈鳞癌61例、C IN 47例、正常子宫颈组织54例,分别采用原位杂交技术检测HPV 16/18感染,用免疫组化技术S-P法检测p53蛋白和M DM 2蛋白。结果HPV 16/18感染率鳞癌组为62.3%(38/61),C IN组为70.2%(33/47),对照组为16.7%(9/54)。C IN组和鳞癌组的HPV 16/18的感染率均显著高于对照组(P值均<0.05)。而C IN组与鳞癌组的HPV 16/18感染率差异无显著性(P>0.05)。C IN组、鳞癌组、对照组的p53阳性率分别为19.1%(9/47)、26.2%(16/61)、3.7%(2/54)。C IN组、鳞癌组、对照组的M DM 2阳性率分别为14.9%(7/47)、26.2%(16/61)、7.4%(4/54)。C IN组和鳞癌组的p53阳性率均显著高于对照组(P值均<0.05),C IN组与鳞癌组比较差异无显著性(P>0.05)。M DM 2阳性率鳞癌组显著高于对照组(P<0.05),而在C IN组和鳞癌组之间、C IN组和对照组之间差异均无显著性(P值均>0.05)。在本实验各组中,p53与M DM 2之间的阳性率均无统计学相关(P值均>0.05)。p53阳性率和HPV 16/18感染之间差异无显著性(P值均>0.05),而M DM 2的阳性率与HPV 16/18感染仅在子宫颈鳞癌组呈正相关(P<0.05)。结论C IN及子宫颈鳞癌与HPV 16/18感染关系密切;p53和M DM 2均参与子宫颈癌及癌前病变的发生,但p53的突变、M DM 2的扩增和过表达均不占主导地位。Objective To investigate the expression of p53 and MDM2 protein,and HPV16/18 infection in squamous cervical carcinoma （SCC） and cervical intraepithelial neoplasia （CIN）. Methods HPV16/18 were detected by in situ hybridization （ISH）, the expression of p53 and MDM2 protein was examined by immunohistochemistry in 61 of SCC, 47 of CIN and 54 of normal cervix （NC）. Results HPV16/18 positive rates in SCC,CIN and NC groups were 62.3%（38/61）, 70. 2 % （33/47） and 16.7 % （9/54）, respectively （SCC vs NC and CIN vs NC, P〈0. 05）. The p53 positive rates in SCC,CIN and NC groups were 26.2%（16/61）,19.1%（9/47） and 3. 7%（2/54）, respectively （SCC vs NC and CIN vs NC,P〈0. 05; SCC vs CIN,P〉0.05）. The MDM2 positive rates in SCC,CIN and NC groups were 26.2% （16/61）, 14.9% （7/47） and 7.4% （4/54）, respectively （CIN vs NC,P〉0.05; SCC vs NC and SCC vs CIN,P〈0.05）. There was no correlation among the positive rates of p53 protein or MDM2 protein with HPV16/18 infection rates（P〉0. 05）, while the MDM2 positive rates was related to HPV16/18 infection rates in SCC group（P〈0. 05）. Conclusions HPV16/18 infection has a strong association with SCC and CIN. p53 and MDM2 proteins may be involved in the development of CIN and SCC.

关 键 词：癌 鳞状细胞/病理学 蛋白质p53/生物合成 宫颈肿瘤/病理学 乳头状瘤病毒 人/致病力 癌基因 免疫组织化学 原位杂交 

分 类 号：R737.33[医药卫生—肿瘤]