补肾益智方对老年性痴呆模型大鼠神经递质释放的影响(英文)  

Effect of bushen yizhi formula on neurotransmitter release in rat models with Alzheimer disease

作　　者：钟振国[1] 刘茂才[2] 赖世隆[2] 高洁[2] 程淑意[2] 

机构地区：[1]广西中医学院第二附属医院神经科学研究所,广西壮族自治区南宁市530011 [2]广州中医药大学老年脑病研究所,广东省广州市510405

出　　处：《中国临床康复》2005年第44期167-170,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:胆碱乙酰基转移酶活性水平的降低和神经递质囊泡膜蛋白的减少是老年性痴呆的主要生化指标,中药补肾益智方具有补肾、益气、生髓、养脑作用,其含药血清能有效地保护由淀粉样蛋白引起的这些改变。目的:观察补肾益智方含药血清能否改善由淀粉样蛋白产物诱导神经细胞显示的老年性痴呆样病理损害,以此评价补肾益智方治疗老年性痴呆的作用。设计:随机对照实验。单位:广西中医学院第二附属医院神经科学研究所,广州中医药大学老年脑病研究所。材料:实验于2003-09/2004-03在广西中医学院新药研究开发中心完成。选取健康清洁级3月龄Wistar大鼠60只,随机分为空白对照组与中药灌胃组,各30只。补肾益智方主要由枸杞、蛇床子、人参、首乌、丹皮、冰片等组成,临用前配置为成药0.176g/mL(生药1.13g/mL)液体。方法:①中药灌胃组每只大鼠每天灌胃成药液1.0～1.5mL,连续灌胃1个月,于末次给药后4h内,5000r/min离心5min分离血清,无菌过滤,56℃灭活补体,即为含药血清。空白对照组给予等量生理盐水灌胃,同样方法提取分离血清。②对于NG108-15细胞,当淀粉样蛋白25～35片段在培养液浓度为5μmol/L或淀粉样蛋白1-42的浓度为200nmol/L时较为合适。③NG108-15细胞以Dulbecco改良的Eagle培养基在37℃,体积分数为0.1的CO2培养箱中培养1d,然后加入浓度为5μmol/L淀粉样蛋白25-35或200nmol/L淀粉样蛋白1-42片段,继续培养1d后,即为老年性痴呆细胞模型。以含50g/L中药灌胃组血清或空白对照组血清的Dulbecco改良的Eagle培养基更换原培养液,仍加入上述浓度的淀粉样蛋白片段以维持老年性痴呆细胞模型,分化培养5d后进行各项指标观察,并设立无淀粉样蛋白片段对照。④利用免疫蛋白印迹检查、免疫放射活性测定及电生理学测定方法观察补肾益智方含药血清处理NG108-15细胞老年性痴呆模型后�BACKGROUND： The decrease of activity level of choline acetyltransferase （ChAT） and reduction of membrane protein of synaptic vesicles are the main biochemical indexes in Alzheimer disease. Traditional Chinese bushen yizhi formula is of the effects of strengthening the kidney, replenishing qi, generating marrow and nourishing the brain, its medicine-containing serum can effectively protect these changes resulting from amyloid protein.OBJECTIVE： To investigate whether the medicine-containing serum of bushen yizhi formula can improve the pathological injury of neurons induced by amyloid protein metabolites in Alzheimer disease, thus to estimate the therapeutic effect of bushen yizhi formula on Alzheimer disease.DESIGN：A randomized and controlled trial. SETTING：Institute of Neuroscience, Second Affiliated Hospital, Guangxi College of Traditional Chinese Medicine, Nanning 530011, Guangxi Zhuang Nationality Autonomous Region, China; Encephalopathic Institute of Guangzhou University of Traditional Chinese Medicine.MATERIALS：The experiment was conducted from September 2003 to March 2004 at the Research and Development Center of New Drugs of Guangxi College of TCM. Totally 60 healthy Wistar rats of clean grade,three months, were at random divided as blank control and bushen yizhi groups, with 30 in each group. The bushen yizhi formula consisted of Fructus Lycii, Fructus Cnidii, Radix Gingeng, Radix Polygoni Multiflori,Cortex Moutan Radicis, Borneolum, etc. The medicine was prepared before use as 0.176 g/Ml liquid（including 1.13 g/Ml rude drugs）.METHODS：① Each of the rats in bushen yizhi group was by garage given 1.0-1.5 Ml prepared liquid per day for consecutive 30 days. Within 4hours after the last administration the serum was isolated at 5 000 r/min for 5 minutes, filtered aseptically, the compliment was inactivated at 56 ℃,then the medicine-containing serum was obtained. The rats in blank control group were given saline of the same volume, and the serum was prepared with the same method. ② For

关 键 词：补肾药 益智 淀粉样Β蛋白 痴呆/中药疗法 神经递质 疾病模型 动物 

分 类 号：R749.1[医药卫生—神经病学与精神病学]