葛根总黄酮对糖尿病合并脑缺血再灌注小鼠的影响  

Effects of Pueraria Lobata Isoflavones on Diabetes Mellitus Combined with Cerebral Ischemic Reperfusion Mouse

作　　者：苗明三[1] 孙丽敏[1] 苗艳艳[1] 

机构地区：[1]河南中医学院,郑州市450008

出　　处：《医药论坛杂志》2005年第23期11-13,16,共4页Journal of Medical Forum

基　　金：河南省高校杰出科研创新人才基金(2001KYCX001);河南省杰出创新人才基金(0221001800)

摘　　要：目的观察葛根总黄酮(PLIS)对小鼠糖尿病性脑缺血再灌注模型脑能量代谢及脑组织的影响。方法采用四氧嘧啶复制小鼠糖尿病模型;用直径约为0.3mm的针灸针与CCAs血管一起结扎,20m in后抽出针灸针的方法复制出脑缺血再灌注模型;通过测定该模型小鼠脑组织中钠钾ATP酶、钙镁ATP酶、LD、LDH的值,测定小鼠脑水肿及观察组织病理学的情况,探讨PLIS对小鼠糖尿病合并脑缺血再灌注模型脑能量代谢及脑组织的影响。结果PLIS可显著提高钠钾ATP酶、钙镁ATP酶活性,降低LD、LDH水平,降低脑含水量,对模型所致神经细胞和胶质细胞损伤有显著的拮抗作用和增强细胞耐缺氧作用。结论PLIS有改善糖尿病性脑缺血小鼠模型脑能量代谢及脑水肿情况,明显改善脑组织的病理情况,有保护脑缺血再灌注损伤作用。Objective To explore the effect of pueraria lobata isoflavones （PLIs） on cerebral energy metabolism and cerebral tissue of the diabetes mellitus combined with cerebral ischemic reperfusion model mice. Methods Establish the diabetes mellitus combined with cerebral ischemic reperfusion mice models by ligating CCAs combined with acupuncture pin which diameter is about 0.3 mm and is taken away after ligating for 20min in the diabetes mellitus mouce models induced by injection with alloxan. We judge the effect of PLIs on cerebral energy metabolism and cerebral tissue of the diabetes mellitus combined with cerebral ischemic reperfusion model mice by measuring the indexes such as Na^＋ - k^＋ - ATPase, Ca^＋＋ - Mg^＋＋ - ATPase, LD and LDH in cerebral tissue, and by analyzing the degree of cerebral edema and the histopatholgical changes of the model mice. Results PLIs significantly enhances the activity of Na^＋ - k^＋ - ATPase, Ca^＋＋ - Mg^＋＋ - ATPase, decreases the amount of LD、LDH and the degree of the cerebral edema, significantly counteract the neural cell and colloid cell, and can improve the ability of anti - anoxia and ameliorate the pathological injury obviously. Conclusion PLIs can obviously ameliorate the cerebral energy metabolism and the condition of cerebral edema, can ameliorate the pathological changes of cerebral tissue and the injury induced by cerebral ischemic combined with reperfusion.

关 键 词：糖尿病性脑缺血小鼠模型 脑能量代谢 脑水肿 组织形态 

分 类 号：R-332[医药卫生]