常见食用菌中转基因成分的多重PCR检测技术模拟  

Detection of genetically modified component in familiar edible fungi by multiplex PCR

作　　者：陈文炳[1] 朱晓南[1] 江树勋[1] 邵碧英[1] 李寿崧[1] 

机构地区：[1]福建出入境检验检疫局,福建福州350001

出　　处：《福建农林大学学报（自然科学版）》2005年第4期478-482,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家质量监督检验检疫总局科研项目(2002IK084;2003IK046);福建省科技厅重大项目(2001H011);福建省青年科技人才创新项目(2001J040)资助

摘　　要：将食用菌转基因研究用的p301-bG1质粒DNA,添加到19种常见食用菌样品中,作为模拟阳性样品,从中提取出DNA用于多重PCR分析,建立了食用菌模拟阳性样品中4个大小分别为165、398、545和600 bp的外源基因(NOS、BAR、GUS与NPTⅡ)的特异性DNA片段的5组二重PCR与2组三重PCR检测方法.Plasmid DNA （p301-bG1） used in transgenic research of familiar edible fungi was added to edible fungi samples as simulated positive samples. Total DNAs were extracted from 19 samples by CTAB method. Multiplex PCR detection method was developed for the target DNA fragments with the size of 165, 398,545 and 600 bp of four transgenes, i.e. NOS, BAR, GUS and NPT- Ⅱ respectively. Five combinations of duplex PCR including NOS-GUS, BAR-GUS, NOS-BAR, NOS-NPT Ⅱ and BAR-NPT Ⅱ , as well as two combinations of triplex PCR including NOS-BAR-GUS and NOS-BAR-NPT Ⅱ , were performed.

关 键 词：食用菌 转基因成分 多重PCR 

分 类 号：Q78[生物学—分子生物学]