气管干细胞分化过程中Shh信号通路的动态观察  

Dynamic changes of sonic hedgehog signaling pathway during tracheal stem cell differentiation

作　　者：廖爱军[1] 贾心善[2] 周莹[3] 

机构地区：[1]中国医科大学附属第二医院血液内科,辽宁沈阳110004 [2]中国医科大学基础医学院病理学教研室 [3]中国医科大学附属第一医院急诊科

出　　处：《中国医科大学学报》2005年第6期500-501,共2页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30170407)

摘　　要：目的:探讨大鼠气管上皮损伤后,气管干细胞增殖分化过程中Shh信号通路的动态变化。方法:应用5-氟尿嘧啶(5-FU)诱发气管上皮损伤,通过RT-PCR检测气管上皮Shh的表达。结果:5-FU作用30 m in后大鼠气管上皮细胞绝大部分脱落,仅残留少量裸核样G0期细胞位于基底膜上。之后细胞数目逐渐增多,经过扁平、立方形态,向纤毛细胞、黏液细胞、基底细胞等分化,去除5-FU后48 h接近恢复假复层纤毛柱状上皮;正常气管上皮及5-FU作用30 m in气管上皮Shh无表达,去除5-FU恢复3 h后Shh轻度表达,6 h后表达增强,12 h达高峰,之后表达减弱,48 h已极微弱。结论:Shh参与了气管干细胞的增殖分化过程。Objective： To study the dynamic changes of sortie hedgehog （Shh） signaling pathway during tracheal stem cell differentiation after tracheal epithelial injury in rats. Methods ：The tracheal epithelial injury was induced by 5-fluorouracil （5-FU）. The expression of Shh in the tracheal epithelium was detected by RT-PCR. Results：Most tracheal epithelial ceils exfoliated 30 minutes after the application of 5-FU, and a few stripped nucleus-like ceils were randomly distributed on the basement membrane. The number of cells on the basement membrane increased gradually after 5-FU was removed, and the squamous and columnar epithelial cells differentiated into ciliated, mucosal, and basal ceils. The epithelial cells differentiated into pseudostratified ciliated columnar epithelial ceils 48 hours after 5-FU was removed. There was no expression of Shh in normal epithelium and injured epithelium 30 minutes after the application of 5-FU. The expression of Shh occurred gradually 3 hours after 5-FU was removed, increased 6 hours later, and reached the peak 12 hours later. Then the expression of Shh decreased and was faint 48 hours later. Conclusion：Shh may be involved in the proliferation and differentiation of tracheal stem cells.

关 键 词：SHH 气管 干细胞 损伤 分化 

分 类 号：R364[医药卫生—病理学]