酒精性肾损害时核转录因子κB的表达及复方中药的干预作用  

Effect of compound Chinese drug on the expression of nuclear factor kappa-B in alcohol-induced kidney damage

作　　者：张健[1] 鞠晓华[1] 王炳元[1] 傅宝玉[1] 

机构地区：[1]中国医科大学附属第一医院消化内科

出　　处：《中国医科大学学报》2005年第6期509-510,共2页Journal of China Medical University

基　　金：辽宁省教育厅B类基金资助项目(20121265)

摘　　要：目的:探讨核转录因子κB(NF-κB)在酒精性肾损害时的表达及复方中药的干预作用。方法:63只大鼠随机分为正常组、酒精组及中药组,按本室方法用乙醇及抗纤复方I号直接灌胃,于4,8,12周末处死大鼠,HE染色、透射电镜观察病理改变;免疫组化方法检测肾组织中NF-κB的表达。结果:中药组同酒精组比较,肾小球系膜增殖、间质局灶性炎性细胞浸润、肾小管上皮扁平化和肾小管扩张等病理改变出现延迟且明显减轻。随着造模时间的延长,酒精组NF-κB表达进行性增强;中药组与酒精组比较明显减弱(P<0.05)。结论:抗纤复方I号能够抑制酒精性肾损害时NF-κB的表达。Objective： To explore the effect of compound Chinese drug on the expression of nuclear factor kappa-B （NF-KB） in experimental alcohol-induced kidney damage. Methods：Sixty-three rats were randomly divided into normal group, alcohol group, and Chinese drug group. Alcohol and compound Chinese drug KX I were lavaged into the stomachs of rats to establish the animal model. The rats in each group were killed 4, 8, and 12 weeks later, separately. The pathological changes were observed with HE staining and transmission electron microscope. The expression of NF-KB was determined by using immunohistochemical method. Results： Hyperplasia of mesangium, interstitial infiltration of inflammatory cells, degeneration of renal tubular epithelial cells, dilatation of tubules, and other changes were observed in alcohol group. The expression of NF-KB gradually increased with the development of alcohol-induced kidney damage. However, the pathological changes and the expression of NF-KB in Chinese drug group appeared later and lighter than those in alcohol group （ P 〈 0.05）. Conclusion：The compound Chinese drug KX Ⅰ can inhibit the expression of NF-KB in alcoholinduced kidney damage.

关 键 词：酒精性肾损害 核转录因子KB 复方中药 

分 类 号：R575.2[医药卫生—消化系统]