阳离子脂质体介导β干扰素基因转染对人胶质瘤细胞的抑制作用  

Inhibitory effect of interferon-β gene transfection mediated by cation liposome on the proliferation of human glioma cell line

作　　者：李光宇[1] 刘薇[1] 吴安华[1] 

机构地区：[1]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《中国医科大学学报》2005年第6期556-557,共2页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30300100)

摘　　要：目的:建立阳离子脂质体载体,介导β干扰素(IFN-β)基因转染人胶质瘤细胞系,观察其生长抑制作用。方法:以反相蒸发法制备含IFN-β的阳离子脂质体载体,转染SK-MG-1胶质瘤细胞系。MTT法观察其生长抑制情况,流式细胞技术检测肿瘤细胞的凋亡。结果:阳离子脂质体介导IFN-β基因转染可抑制SK-MG-1细胞的生长,抑制作用强于IFN-β蛋白。IFN-β蛋白和基因转染可以诱发SK-MG-1细胞的凋亡。结论:阳离子脂质体可作为一种有效的载体应用于胶质瘤的基因治疗中。IFN-β可抑制胶质瘤细胞系SK-MG-1的生长,诱发凋亡。Objective： To construct cation liposome vector and transfect interferon-β （IFN-β） gene into human glioma cell line with it, and to observe the inhibitory effect of IFN-β on the proliferation of the cell line. Methods： The cation liposome vector containing IFN-β was constructed by reverse-phase evaporation and was used to transfect human glioma cell line SK-MG-1. The inhibitory effect of IFN-β on the proliferation of the cell line was measured by MTr method, and the apoptosis of glioma cells was determined by flow cytometry. Results： IFN-β gene transfection inhibited the proliferation of human glioma cell line SK-MG-1 and induced the apoptosis of glioma cells, Conclusion： Cation liposome is an effective vector used to transfect IFN-β gene into human glioma cell line. IFN-β can inhibit the proliferation of human glioma cell line SK-MG-1 and induce the apoptosis of the glioma cell.

关 键 词：胶质瘤 阳离子脂质体 干扰素Β 凋亡 

分 类 号：R739.41[医药卫生—肿瘤]