耦合固定化技术在天冬氨酸转氨酶反应体系中的应用  

Application of coupling immobilization to enzymatic transformation system of aspartate aminotransferase

作　　者：胡永红[1] 汤天羽[1] 蒋昊海[1] 周华[1] 沈树宝[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《工业微生物》2005年第4期6-8,14,共4页Industrial Microbiology

基　　金：江苏省自然科学基金(No.BK2002017);江苏省高校自然科学基金(01KJB530003)资助

摘　　要：对卡拉胶与明胶形成的耦合固定化体系进行了优化,并探讨海藻糖和金属离子(Mg2+)等辅助因子在该体系中对酶转化过程的影响。结果表明将该法应用于L-苯丙氨酸转化体系中,保护了天冬氨酸转氨酶的活性,显著提高了酶活回收率,天冬氨酸转氨酶的活力回收达到了93.6%,较文献报道有明显提高。The optimal conditions and the effects of trehalose and Mg^2＋ in the carrageenan and gelatin of coupling immobilization were studied. The integrated system was applied in L-phenylalanine production process. The reclaimable ratio of the aspartate aminotransferase of coupling immobilization was obviously better than that of single immobilization. The recovery of aspartate aminotransferase was about 93.6 % , higher than that of the literature reported.

关 键 词：固定化 酶转化 耦合技术 

分 类 号：O629.8[理学—有机化学]