兔防御素基因酵母表达的研究  

Construction and Transformation of Recombinant for the Rabbit Defensin NP2 Gene Expression in Yeast

作　　者：李丽[1] 王川[1] 李再新[1] 

机构地区：[1]四川理工学院生物工程系,四川自贡643000

出　　处：《四川理工学院学报（自然科学版）》2005年第4期79-81,共3页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

摘　　要：防御素是由动植物体内产生的一种多肽类抗菌、抗病毒活性物质,本研究根据巴斯德毕赤 酵母偏爱密码子的特性,设计兔防御素NP2基因,并构建巴斯德毕赤酵母的表达质粒pGAPZα-NP2,通 过LiCl法转入巴斯德毕赤酵母中,筛选和检测结果表明,兔防御素NP2基因已经成功地转入巴斯德毕 赤酵母,并得到表达。该实验可为兔防御素NP2抗菌抗病毒的研究和开发奠定理论和物质基础。Defense is a kind of cation polypeptide and anti-bacterium activity materials, which is generally produced in many animal and plant lives. In this experiment, the sequence of rabbit defensin NP2 gone is made according to the preferential codons for Pichia pastoris yeast in translation. The expression vector for Pichia pastoris yeast is designed with rabbit defensin NP2 gene, and constructed pGAPZα-NP2. with the method of LiCl to transform pGAPZα-NP2 into the Pichia pastoris yeast, the result of PCR examining indicates that rabbit dcfensin NP2 gene has existed in Pichia pastoris yeast and has been expressed success full.

关 键 词：兔防御素 毕赤酵母 表达 

分 类 号：Q784[生物学—分子生物学]