Characterization of a new mutant allele of the Arabidopsis Flowering Locus D (FLD) gene that controls theflowering time by repressing FLC  

作　　者：CHEN Ruiqiang ZHANG Suzhi SUN Shulan CHANG Jianhong ZUO Jianru 

机构地区：[1]Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2]Graduate School, Chinese Acadamy of Sciences, Beijing 100049, China

出　　处：《Chinese Science Bulletin》2005年第23期2701-2706,共6页

基　　金：This work was supported by the Chinese Academy of Sciences,the National Natural Science Foundation of China(Grant No.30125025&30221002);The Ministry of Science and Technology of China

摘　　要：Flowering in higher plants is controlled by both the internal and environmental cues. In Arabidopsis, several major genetic loci have been defined as the key switches to control flowering. The Flowering Locus C (FLC) gene has been shown in the autonomous pathway to inhibit the vege-tative-to-reproductive transition. FLC appears to be re-pressed by Flowering Locus D (FLD), which encodes a com-ponent of the histone deacetylase complex. Here we report the identification and characterization of a new mutant allele fld-5. Genetic analysis indicates that fld-5 (in the Was-silewskija background) is allelic to the previously character-ized fld-3 and fld-4 (in the Colombia-0 background). Genetic and molecular analyses reveal that fld-5 carries a frame-shift mutation, resulting in a premature termination of the FLD open reading frame. The FLC expression is remarkably in-creased in fld-5, which presumably attributes to the ex-tremely delayed flowering phenotype of the mutant.Flowering in higher plants is controlled by both the internal and environmental cues. In Arabidopsis, several major genetic loci have been defined as the key switches to control flowering. The Flowering Locus C （FLC） gene has been shown in the autonomous pathway to inhibit the vegetative-to-reproductive transition. FLC appears to be repressed by Flowering Locus D （FLD）, which encodes a component of the histone deacetylase complex. Here we report the identification and characterization of a new mutant allele f/d-5. Genetic analysis indicates that fld-5 （in the Wassilewskija background） is allelic to the previously characterized fld-3 and fld-4 （in the Colombia-0 background）. Genetic and molecular analyses reveal that fld-5 carries a frame-shift mutation, resulting in a premature termination of the FLD open reading frame. The FLC expression is remarkably increased in fld-5, which presumably attributes to the extremely delayed flowering phenotype of the mutant.

关 键 词：阿布属 开花基因位点 突变等位基因 植物遗传学 开花时间控制 

分 类 号：Q943.2[生物学—植物学]