苯并（a）芘对淋巴细胞钙稳态的影响及其与免疫抑制的关系  

The Effect of benzo(a) pyrene on lymphocyte calclum homeostasis and its relation to immunosup-pression

作　　者：张海洲[1] 张铣[1] 薛彬[1] 

机构地区：[1]北京医科大学卫生毒理学教研室

出　　处：《中华劳动卫生职业病杂志》1996年第4期200-203,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金

摘　　要：利用一次腹腔注射的染毒方法观察了苯并（ａ）在（ＢａＰ）对小鼠脾淋巴细胞钙稳态的影响，并初步探讨了其与免疫抑制的关系。结果表明，ＬＡＣＡ雌性小鼠一次腹腔注射ＢａＰ２００ｍｇ／ｋｇ后第３天和第６天，胸腺和脾脏明显萎缩，与对照组比其相对重量分别下降４６．０％～４９．２％和２８．７％～３３．０％；全脾细胞数明显减少，同时刀豆素Ａ（ＣｏｎＡ）刺激的淋巴细胞增殖反应也受到明显抑制，抑制率为２１．１％～２７．５％；淋巴细胞内游离钙浓度（［Ｃａ￣(２＋)］ｉ）在染毒后第３天明显升高，第６天明显下降；不同剂量ＢａＰ染毒后第３天淋巴细胞内［Ｃａ￣(２＋)］ｉ与染毒剂量呈明显正相关（ｒ＝０．９８５３，Ｐ＜０．０１）；第６天［Ｃａ￣(２＋)］ｉ与染毒剂量呈明显负相关（ｒ＝－０．９４７３，Ｐ＜０．０１）。此外，还观察到钙离子载体Ａ＿(２３１８７)使正常小鼠脾淋巴细胞［Ｃａ￣(２＋)］ｉ明显升高时，淋巴细胞增殖反应受到明显抑制。结果提示，ＢａＰ可明显影响小鼠脾淋巴细胞的钙稳态，而钙稳态的失衡与ＢａＰ引起的免疫抑制可能有密切的关系。Femal LACA mice were exposed to BaP by a single intraperitoneal injection to investi-gate the effect of BaP on calcium hemeostasis of splenic lymphocyte and the relationship between thiseffect and immunossuppression induced by BaP.A significant decrease in relative weights of the thymus(46.0%～49.2%)and spleen(28.7%～33.5%)was observed in mice exposed to 200 mg/kg BaP 3 and6 days after exposure. At the same dose,the number of splenocytes was significantly decreased (24%～81%)and the splenic lymphocyte proliferation by ConA was also inhibited (21.1%～27.5%)signifi-cantly in exposed mice 3,6,and 9 days later. On day 3 and day 6 after injection the intracellular free cal-cium concentration of lymphocyte was increased (r=20.985 3,P<0. 01)and decreased (r=-0.947 3,P<0. 05)in a dose-dependent manner.The intracellular free calcium level of splenic lymphocytes in nor-mal mice was also elevated by calcium ionophore A_(23187),whereas the proliferation by ConA was de-creased significantly,The results demonstrated that BaP caused disturbance of calcium homeostasis,which may relate with immunosuppression induced by BaP.

关 键 词：苯并(A)芘 钙稳态 免疫抑制 淋巴细胞 

分 类 号：R363.13[医药卫生—病理学] R392.12[医药卫生—基础医学]