烯唑醇、三唑醇和腈菌唑的GC和HPLC分析方法  

Study on the Quantitative Analysis Method of Diniconazole, Triadimenol and Myclobutanil by GC and HPLC

作　　者：慕卫[1] 陈召亮[1] 杨连来[1] 孙作洋[1] 李燕平[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《现代农药》2005年第6期17-19,共3页MODERN AGROCHEMICALS

摘　　要：采用气相色谱法和液相色谱法两种分析方法分别对烯唑醇、三唑醇和腈菌唑进行定性定量分析。气相色谱法中分别以二十烷和邻苯二甲酸二丁酯为内标物,色谱柱都采用3%OV-101chromosorbAWDMCS150–177μm,1m×3mm玻璃柱,柱温为195℃,汽化温和检测温均为230℃,烯唑醇、三唑醇和腈菌唑的回收率分别为99.5%～101.0%,99.5%～101.2%和98.9%～99.7%,方法变异系数分别为0.44%,0.32%和0.21%。高效液相色谱法中使用Nova-PaKC18250mm×4.6mm色谱柱,以甲醇–水(体积比85:15)为流动相,检测波长为252nm、223nm和223nm。烯唑醇、三唑醇和腈菌唑的回收率分别为98.9%～100.4%,99.4%～100.9%和99.5%～101.2%,方法变异系数分别为0.65%,0.41%和0.47%。The quantitative analysis method ofdiniconazole, triadimenol and myclobutanil with GC and HPLC was studied, In gas chromatographic method, a 3% OV - 101 on Chromosorb AW DMCS 150- 177μm, 1 m×3 mm （i.d.） glass column was used at column temperature of 195℃ and detector temperature of 230℃, The internal standard was n-eicosane and dibutyl o-phthalate respectively. This method had a recovery of 99.1%～ 100.3%, 98.5%～ 99.8% and 99.3%～ 100.7%, their coefficients of variation were 0.44%, 0.32% and 0.21%, respectively. Operating conditions by HPLC were C18 column （250 mm×4.6 mm）, V（methanol）：V（water） = 85：15 as mobile phase, spectrum length UV - 252 nm, 223 nm and 223 nm. This method had a recovery of 99.1%～100.7%, 98.9%～99.6% and 99.9%～101.2% and their coefficient of variation were 0.65%, 0.41% and 0.47% for diniconazole, triadimenol and myclobutanil respectively.

关 键 词：烯唑醇 三唑醇 腈菌唑 分析 GC HPLC 

分 类 号：TQ455[化学工程—农药化工]