影响茎用芥菜愈伤组织诱导和植株再生的因素  

Factors Affecting Callus Induction and Plant Regeneration of Tuber Mustard (Brassica juncea Coss. var. tumida Tsen et Lee)

作　　者：陈利萍[1] 徐春霞[1] 李春顺[1] 

机构地区：[1]浙江大学园艺系,杭州310029

出　　处：《植物生理学通讯》2005年第6期758-760,共3页Plant Physiology Communications

基　　金：浙江省自然科学基金项目(302340)

摘　　要：茎用芥菜子叶培养在MS+0.25mg·L-1NAA+0.5mg·L-16-BA+0.25mg·L-12,4-D培养基上,可获得较高质量的愈伤组织。愈伤组织培养在MS+1￣2mg·L-16-BA+0.2mg·L-1NAA培养基上分化频率为8.3%,而在加有羧苄青霉素和头孢霉素的培养基上,最高分化频率可达52.4%。将获得的再生植株转移到1/2MS+0.1mg·L-1NAA的生根培养基中,可获得完整植株。When the cotyledons of tuber mustard （Brassica juncea Coss. var. tumida Tsen et Lee） were cultured on MS medium containing with 0.25 mg·L^-1 NAA, 0.5 mg·L^-1 6-BA and 0.25 mg·L^-1 2,4-D, the high quality calli were obtained. The differentiation rate of these calli on MS medium containing 1-2 mg·L^-1 6-BA and 0.2 mg·L^-1 NAA was 8.3%. When the cefotaxime or carbenicillin was.added into MS medium containing 1-2 mg·L^-1 6-BA and 0.2 mg·L^-1 NAA, the maximum differentiation rate reached 52.4%. After all the regenerated shoots were transplanted on 1/2MS medium added 0.1 mg·L^-1 NAA （rooting medium）, the entire plantswere obtained.

关 键 词：茎用芥菜 愈伤组织 分化 抗生素 植株再生 

分 类 号：S637.3[农业科学—蔬菜学]