葛根素注射液对家兔肝缺血再灌注损伤的防治作用  

Protective effects of puerarin injection on hepatic ischemia-reperfusion injury in rabbits

作　　者：许冬武[1] 王万铁[1] 徐正祄[2] 王卫[3] 朱传新 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035 [2]温州医学院,浙江温州325035 [3]温州医学院温州市疾病控制中心,浙江温州325035

出　　处：《温州医学院学报》2005年第6期440-442,共3页Journal of Wenzhou Medical College

基　　金：浙江省卫生厅科研基金(98A087)

摘　　要：目的：观察葛根素注射液对家兔肝缺血再灌注损伤(HIRI)的防治作用。方法：制备家兔HIRI模型,30只健康家兔随机分为3组：假手术组(S组)10只,缺血再灌注组(IR组)10只,葛根素组(Pur组)10只。观察肝组织及血中超氧化物歧化酶(SOD)活性、黄嘌呤氧化酶(XO)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、谷丙转氨酶(ALT)活性、丙二醛(MDA)含量及肝细胞形态学变化。结果：肝缺血再灌注后,葛根素组与缺血再灌注组比较,血浆中SOD、XO、MDA、GSH-Px、ALT差异均有显著性或非常显著性(P＜0.05或P＜0.01)；肝细胞形态学异常改变明显减轻。结论：葛根素注射液通过拮抗脂质过氧化反应而对HIRI具有良好的防治作用。Objectlve：To explore the protective effects of Puerarin injection on hepatic ischemia-reperfusion injury （HIRI）. Methods： Using a HIRI model, 30 rabbits were randomly divided into 3 groups： sham-operation group （n=10）, ischemia-reperfusion group （n=10）, and Puerarin group（n=10）. Superoxide dismutase （SOD） activity, xanthine oxidase （X0） activity, glutathione peroxidase （GSH-Px） activity,alanine aminotransferase（ALT）activity, and malondialdehyde （MDA） content in both serum and hepatic tissue were measured in various groups, and the morphological changes in hepatocytes were also observed during HIRI. Results： Compared to IR group respectively in the Puerarin treated group, serum SOD XO GSH-Px ALT activities and MDA contents significantly changed at 45 minute ischemia and 45 minuter reperfusion, the abnormal changes of all above parameters were significant differences（P〈 0.05 or P〈 0.01） and hepatocyte damage in morphologically was remarkably ameliorated. Conclusions： Puerarin injection has notable protective effects on HIRI. by scavenging oxygen free radicals and anti-lipid peroxidation reaction.

关 键 词：葛根素注射液 缺血再灌注损伤 肝脏 氧自由基 

分 类 号：R363.2[医药卫生—病理学]