大鼠尾加压素Ⅱ收缩大鼠肺主动脉与钙离子通道相关  

Correlation between rat urotensin-Ⅱ induced contraction of the main trunk of pulmonary artery and Ca^(2+) channel

作　　者：薛必成[1] 陈少贤[1] 龚永生[2] 徐正祄[3] 王良兴[1] 范小芳[2] 胡良刚[3] 

机构地区：[1]温州医学院第一附属医院呼吸内科,浙江温州325000 [2]温州医学院肺心病研究室,浙江温州325000 [3]温州医学院病理生理学教研室,浙江温州325000

出　　处：《温州医学院学报》2005年第6期446-448,共3页Journal of Wenzhou Medical College

基　　金：浙江省教育厅科研基金(20020468)

摘　　要：目的：研究大鼠尾加压素Ⅱ(U-Ⅱ)收缩大鼠离体肺主动脉环与钙离子通道的关系。方法：从雄性Sprague-Dauley大鼠中分离出肺主动脉,切成3～4mm的血管环。用大鼠U-Ⅱ(30 nmol／L)预收缩血管达平台期后,加入钙离子通道阻断剂尼群地平,制备尼群地平(0.1～30μmol／L)浓度-效应舒张曲线,分别计算EC_(50)和Emax。结果：尼群地平可浓度依赖性舒张大鼠U-Ⅱ预收缩的肺主动脉环[-log(EC_(50))=5.86±0.30,Emax=(73.73±15.40)％,以30 nmol／L U-Ⅱ的收缩幅度为100％]。结论：钙离子通道的激活参与大鼠U-Ⅱ收缩大鼠肺主动脉效应。Objective： To investigate the relationship between rat urotensin- Ⅱ-induced contraction in rat main trunk of the pulmonary arteries and the activation of Ca^2＋ channel. Methods： The main trunk of the pulmonary artery was dissected in the male Sprague-Dawley rat and cut into arterial ring in 3～4 mm width. Inhibitor of Ca^2＋, nitrendipine （0.1 ～ 30 μmol/L） was added into the incubation medium after rat U- Ⅱ （30 nmol/L） induced contraction reached plateau to prepare the relaxant concentration-response curves. Their EC50 and Emax were calculated, respectively. Results： Nitrendipine could relax concentration-dependently the arterial rings of the rat U- Ⅱ precontracted arteries [-log[EC50]=5.86± 0.30, Emax=（73.73±15.40%） of the response to 30 nmol/ L rat U-Ⅱ ]. Conclusion： The activation of Ca^2＋ participates in the response of rat U-Ⅱ-induced contraction of the main trunk of the pulmonary artery.

关 键 词：尾加压素Ⅱ 钙离子通道 尼群地平 肺主动脉 

分 类 号：R363[医药卫生—病理学]