乙肝病毒相关性肝病毒物代谢酶基因多态性研究  

Study on genetic polymorphisms of xenobiotica metabolizing enzymes in hepatitis B virusassociated hepatic diseases

作　　者：张友才[1] 邓长生[2] 朱尤庆[2] 

机构地区：[1]温州医学院第一附属医院感染内科,浙江温州325000 [2]武汉大学中南医院消化内科,湖北武汉430071

出　　处：《温州医学院学报》2005年第6期464-467,共4页Journal of Wenzhou Medical College

摘　　要：目的：探讨谷胱甘肽硫转移酶(GST)M1、T1及N-乙酰化转移酶1(NAT1)基因多态与肝炎肝硬化、乙肝相关性肝癌的易感性。方法：采用多重PCR和PCR-RFLP技术,检测60例肝细胞肝癌(病例组),66例肝炎肝硬化(肝硬化组)和73例乙肝标志物阴性的健康人(对照组)GSTM1、GSTT1和NAT1基因多态性。结果：GSTM1空白基因型在对照组中的频率显著低于肝癌组(x^2=7.16,P＜0.01),GSTT1空白基因型、NAT1快代谢型在三组研究对象间的频率差异均无显著性；GSTM1空白／GSTT1空白／NAT1快代谢联合基因型在肝癌组中的频率显著高于对照组(x^2=15.98,v=7,P＜0.05),具该联合基因型且HBV阳性的个体患肝癌的危险性升高了2.5倍(OR=2.49,95％CI=1.12-5.53)。结论：GSTM1空白基因型与肝癌的发生有关,GSTM1空白／GSTT1空白／NAT1快代谢联合基因型且HBV阳性的个体患肝癌的危险性显著升高。Objective： To analyze the association of genetic polymorphisms of Glutathione-Stransferase （GST） M1, T1 and N-acetyltransferase I（NAT1） with the hepatitis B virus-associated liver cirrhosis and hepatocellular carcinoma. Methods： Using the method of multiple PCR and PCRRFLP, the genetic polymorphisms of the GSTM1, GSTT1 and NAT1 genes of 73 healthy controls with HBV markers negative and 60 hepatocellular carcinoma and 66 hepatitis B virus-associated liver cirrhosis patients were studied. Results：The frequency of GSTM1 null genotype was significantly fewer in the controls than that of hepatocellular carcinoma patients （x^2=7.16, P〈 0.01）, there were not significant differences of the frequency of GSTT1 null genotype and genotypes containing NATI＊10 between the controls and hepatitis B virus-associated liver cirrhosis or hepatocellular carcinoma. The frequency of combined genotypes with GSTM1 and GSTT1 null genotype/genotypes containing NATI＊10 simultaneously was significantly more common among hepatocellular carcinoma patients when compared with the controls （x^2=15.98, v=7, P〈 0.05）, individuals carrying this combined genotype had more than 2.5-fold risk for developing hepatocellular carcinoma when compared with the individuals carrying other genotypes after they infected hepatitis B virus （odds ratio, OR=2.49, 95% confidence interval, CI=1.12-5.53）. Conclusions：There maybe exist some association GSTM1 null genotype with the hepatocellular carcinoma, the individual carrying combined genotypes with GSTM1 and GSTT1 null genotype/genotypes containing NATI＊10 simultaneously with hepatitis B virus infectious has significantly increased risk of hepatocellular carcinoma.

关 键 词：肝炎病毒 乙型 肝癌 谷胱甘肽转移酶 N-乙酰化转移酶1 

分 类 号：R735.7[医药卫生—肿瘤]