透性化细胞海藻糖合酶的制备及其性质研究  

Preparation and Characterization of Permeabilized Cells in Relation to Trehalose Synthase Activity

作　　者：张峻[1] 陈晓云[1] 齐欣[1] 陈颖[1] 魏雪生[1] 

机构地区：[1]天津市园艺工程研究所,天津300384

出　　处：《南开大学学报（自然科学版）》2005年第5期93-96,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市自然基金资助项目(033605411)

摘　　要：研究了亚栖热菌CBS-01菌株细胞的渗透处理工艺,并对所得透性化细胞海藻糖合酶的性质进行了考察.结果表明:以2%的甲苯为渗透剂,对10%浓度的菌悬液,50℃渗透处理30～60 min即得透性化细胞海藻糖合酶.该透性化细胞酶的最适反应pH为6.2～7.2,最适反应温度为50～65℃;50℃时,催化麦芽糖生成海藻糖的转化率为70%.The permeabilization of Meiothermus sp. CBS-01 cells and characterization of the obtained permeabilized cells in relation to trehalose synthase acfivity was studied. A proper process was achieved with 10%0 cell suspension treated by 2% toluene for 30 min to 60 rain at 50 ℃. The permeabilized cells exhibited optimum trehalose synthase activity at 50 ℃ to 65 ℃ and pH 6.2 to 7.2. The yield of trehalose from maltose by the permeabilized cells was 70% at 50℃.

关 键 词：亚栖热菌 透性化细胞 海藻糖合酶 性质 

分 类 号：Q936[生物学—微生物学]