快速响应温敏凝胶及其在生物分离中的应用研究  

Fast Responsive Temperature-sensitive Hydrogel and Its Application on Bioseparation

作　　者：冯霞[1] 陈莉[1] 董晶[1] 李博林[1] 

机构地区：[1]天津工业大学材料科学与化学工程学院,天津300160

出　　处：《南开大学学报（自然科学版）》2005年第6期34-40,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金资助项目(20174027);天津市自然科学基金资助项目(033610511)

摘　　要：采用减小尺寸与加入C aCO3粒子作致孔剂两种方法,研究了如何提高共聚温敏凝胶P(N IPA-co-GM A-D ex)的响应速度.考察了不同尺寸和多孔凝胶的退溶胀动力学、再溶胀动力学,实验结果表明:减小凝胶尺寸和加入致孔剂对提高凝胶的温度响应速度是有效的.研究了P(N IPA-co-GM A-D ex)凝胶对牛血清蛋白(BSA)的浓缩分离效果,发现P(N IPA-co-GM A-D ex)凝胶对蛋白质溶液有较好的浓缩分离能力.在实验的基础上,设计了一套用于蛋白质提纯的机械模型,测试了其对蛋白质溶液的分离能力,证实了机械模型的可行性.The responsive rate, reswelling and deswelling kinetics of the temperature-sensitive copolymerized P（NIPA-co-GMA-Dex） hydrogels were investigated by decreasing the size of hydrogels and using CaCO3 particles as pore-foaming agent. The results showed that the responsive rate could be increased by decreasing the size of hydrogel and adding CaCO3 particles as pore-foaming agent. Studies on the concentrating effect of the P（NIPA-co-GMA-Dex） hydrogel to the BSA solution show that P（NIPA：co-GMA-Dex） hydrogels can concentrate BSA solution effectively, based on which, a set of mechanical appliance of gel-extraction separation of BSA was designed and its concentrating effect was tested.

关 键 词：N-异丙基丙烯酰胺 葡聚糖 水凝胶 温度敏感 致TLN 生物分离 机械模型 

分 类 号：TQ311[化学工程—高聚物工业]