正丙醇、铜离子、烟酰胺对红霉素合成的作用  

Effects of Propanol,Cu^(2+) and Niacinamide on Biosynthesis of Erythromycin

作　　者：刘勇[1] 叶蕊芳[1] 郑黎[1] 李晓勇 韩素芳 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]河南天方药业有限公司,河南驻马店463600

出　　处：《华东理工大学学报（自然科学版）》2005年第6期808-811,共4页Journal of East China University of Science and Technology

摘　　要：在摇瓶培养过程中,发酵初期加入正丙醇可使红霉素的效价提高49.31%,正丙醇提高了丙酸激酶的比活力,表现出对该酶诱导作用,而对丙酰CoA合成酶比活力无影响。加入铜离子和烟酰胺可使红霉素效价分别提高24%和17%。用HPLC测定发酵液中有机酸含量,分析测定结果认为发酵过程可分为3个阶段:积累期、转折期和生产期。Addition of propanol during erythromycin fermentation with in shake flask improved the titer by 49.3%. Propanol was found to increase the specific activity of propionate kinase, but it had no influence on the specific activity of propionyl-CoA synthetase. Addition of Cu^2＋ and niacinamide, increased erythromycin titer by 24% and 17%, respectively. Using HPLC, organic acid in the broth were measured; it was suggested that the fermentation process can be divided into three phases, accumulative phase, transitional phase and production phase. In production phase, the productivity of erythromycin was the highest.

关 键 词：红霉素 正丙醇 烟酰胺 铜离子 合成 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]